CUSTOM ITEM: HPLC Column, RP C18, Wide Pore, 5um, 4.0mm ID x 100mm Length, 300A. Cogent Brand. 1 EA.
Additional Info:
The 68518‑10P‑4‑3M Cogent RP C18 Wide Pore HPLC Column is a durable, reversed‑phase column designed for enhanced separation of large molecular weight analytes, combining 5 µm particles with a 300 Å wide‑pore structure.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Consistent, reproducible chromatography
The 300 Å pore size allows large molecules to fully penetrate the stationary phase, eliminating restricted diffusion and poor recovery seen with standard 100 Å columns.
100 mm Length – Increased Resolution for Biomolecules
Higher resolving power than 50 mm columns
Improved separation of proteins, peptides, and complex biomolecules
Increased peak capacity for multi‑component samples
Ideal for LC‑MS workflows requiring enhanced resolution
This format is selected when greater separation is needed without moving to longer (150 mm) columns.
4.0 mm ID – Solvent‑Saving Analytical Performance
Comparable performance to 4.6 mm ID columns
Reduces mobile phase consumption and waste
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.9–1.1 mL/min
Benefits:
Lower solvent usage
Reduced operating cost
Compatible with standard HPLC systems
Flow Rate & System Requirements (4.0 mm ID, 5 µm, 300 Å)
✅ Operates under standard high‑pressure HPLC conditions ✅ Provides lower backpressure than 3 µm and 1.8 µm columns, while remaining a high‑pressure analytical column
Suitable for conventional HPLC systems
Tolerant of:
Higher flow rates
Viscous mobile phases
Routine and extended analytical methods
Why Choose Wide Pore (300 Å) C18
Designed for Large Molecules
Enables efficient separation of:
Proteins
Peptides
Oligonucleotides
Eliminates mass transfer limitations of 100 Å phases
Improved Recovery & Peak Shape
Better analyte penetration and interaction
Improved peak symmetry and reproducibility
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
RP C18 (5 µm) Performance Advantages
High‑Pressure Robustness & Reliability
Designed for standard high‑pressure HPLC systems
Lower backpressure than smaller particle columns
Ideal for rugged, long‑life analytical workflows
Reliable Hydrophobic Retention
Strong retention for hydrophobic and neutral compounds
Effective for lipids, fatty acids, proteins, and peptides
Provides greater retention than C8 phases
Method Development Flexibility
Ideal for screening and early‑stage method development
Suitable for USP L1 wide‑pore applications
Flexible across diverse analyte classes
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for polar and basic analytes
High Purity Silica
Consistent chromatographic results
Reduced run‑to‑run variability
Durable & Cost‑Efficient
Longer column lifetime vs smaller particle sizes
Reduced system strain
Lower maintenance frequency
Typical Applications
Protein and peptide separations
Oligonucleotide analysis
Biomolecule LC‑MS workflows
Lipids and hydrophobic compounds
Long‑chain fatty acids
Complex mixtures requiring increased resolution
Important Usage Notes
Designed for large molecule separations using 300 Å pore media
Operates under standard high‑pressure HPLC conditions
Compatible with standard analytical systems
Ideal when robustness and biomolecule compatibility are prioritized over maximum efficiency
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable