CUSTOM ITEM: HPLC Column, RP C18, 5um, 4.0mm ID x 100mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 68518‑10P‑4 Cogent RP C18 HPLC Column is a durable, reversed‑phase column designed for reliable analytical performance, featuring 5 µm particles and fully double end‑capped C18 bonding.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) chemistry, this stationary phase:
Uses complete end‑capping to minimize silanol interactions
Improves peak shape for polar and basic analytes
Provides consistent, reproducible chromatography
This column is ideal as a USP L1 alternate column and a dependable option for routine analytical methods and method development.
100 mm Length – Increased Resolution
Higher resolving power compared to 50 mm columns
Improved separation of closely related compounds
Increased peak capacity for complex samples
Ideal when shorter columns do not provide sufficient resolution
This configuration balances analysis time with improved separation performance.
4.0 mm ID – Solvent‑Saving Analytical Performance
Comparable chromatographic behavior to 4.6 mm ID columns
Reduces mobile phase consumption and waste
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.9–1.1 mL/min
Benefits:
Lower solvent consumption
Reduced operating cost
Compatible with standard HPLC systems
Flow Rate & System Requirements (4.0 mm ID, 5 µm)
✅ Operates under standard high‑pressure HPLC conditions ✅ Produces lower backpressure than 3 µm and 1.8 µm columns, while remaining a high‑pressure analytical column
Suitable for conventional HPLC systems
Tolerant of:
Higher flow rates
Viscous mobile phases
Extended gradients
Why Choose RP C18 (5 µm)
High‑Pressure Robustness & Reliability
Designed for standard high‑pressure HPLC systems
Lower backpressure compared to smaller particle columns
Ideal for rugged, long‑lifetime analytical workflows
Reliable Hydrophobic Retention
Strong reversed‑phase retention for hydrophobic and neutral compounds
Excellent for long‑chain fatty acids
Provides greater retention than C8 phases
Method Development & USP Compatibility
Ideal for new method development and screening
Suitable for USP L1 methods using 5 µm particles
Reliable alternate or replacement column
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for basic analytes
High Purity Silica
Consistent chromatographic results
Reduced variability across runs
Durable & Cost‑Efficient
Longer column lifetime vs smaller particle sizes
Reduced system strain
Lower maintenance and replacement frequency
Typical Applications
Pharmaceutical and QC analysis
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Method development and optimization
Routine analytical HPLC workflows
USP L1 reversed‑phase methods
Important Usage Notes
Operates under standard high‑pressure HPLC conditions
Compatible with conventional analytical systems
Ideal for applications requiring increased resolution with maximum robustness
Preferred when reliability and system compatibility outweigh maximum efficiency
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable