Catalog Number - 68518-10P-3M

The 68518‑10P‑3M Cogent RP C18 Wide Pore HPLC Column is a robust, reversed‑phase column designed for enhanced separation of large molecular weight analytes, combining 5 µm particles with a 300 Å wide‑pore structure to deliver reliable retention, improved recovery, and consistent chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and high‑MW analytes

• Consistent and reproducible chromatography

The 300 Å pore size allows large molecules to fully access the stationary phase, eliminating restricted diffusion and reduced efficiency seen with standard 100 Å columns.

100 mm Length – Increased Resolution for Biomolecules

• Higher resolving power compared to 50 mm columns

• Improved separation of proteins, peptides, and complex biomolecule mixtures

• Increased peak capacity for multi‑component samples

• Ideal for analytical HPLC and LC‑MS workflows requiring greater resolution

This format is selected when 50 mm columns are too fast but insufficient in resolution.

4.6 mm ID – Standard Analytical Performance

• Industry‑standard HPLC column format

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong detector response

• High reproducibility

• Straightforward method transfer across labs

Flow Rate & System Requirements (4.6 mm ID, 5 µm, 300 Å)

✅ Operates under standard high‑pressure HPLC conditions ✅ Produces lower backpressure than 3 µm and 1.8 µm columns, while remaining a high‑pressure analytical column

• Compatible with standard HPLC systems

• Tolerant of:

  • Higher flow rates

  • Viscous mobile phases

  • Longer analytical runs

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Enables effective separation of:

  • Proteins

  • Peptides

  • Oligonucleotides

• Eliminates mass transfer limitations of 100 Å stationary phases

Improved Recovery & Peak Shape

• Better analyte penetration into the stationary phase

• Enhanced peak symmetry and reproducibility

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (5 µm) Performance Advantages

High‑Pressure Robustness & Reliability

• Designed for standard high‑pressure HPLC systems

• Lower backpressure relative to smaller particle sizes

• Ideal for rugged, long‑lifetime analytical methods

Reliable Hydrophobic Retention

• Strong retention for hydrophobic and neutral compounds

• Effective for lipids, fatty acids, proteins, and peptides

• Provides greater retention than C8 phases

Method Development Flexibility

• Ideal for method development and optimization

• Suitable for USP L1 wide‑pore applications

• Flexible across a wide range of analytes

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Better performance for polar and basic analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between runs

Durable & Cost‑Efficient

• Longer column lifetime vs smaller particle sizes

• Reduced system wear

• Lower maintenance frequency

Typical Applications

• Protein and peptide separations

• Oligonucleotide analysis

• Biomolecule HPLC and LC‑MS workflows

• Lipids and hydrophobic compounds

• Long‑chain fatty acids

• Complex mixtures requiring increased resolution

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Operates under standard high‑pressure HPLC conditions

• Compatible with conventional analytical systems

• Ideal when greater resolution is needed while maintaining robust performance