CUSTOM ITEM: HPLC Column, RP C18, 5um, 3.0mm ID x 100mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 68518‑10P‑3 Cogent RP C18 HPLC Column is a durable, reversed‑phase column designed for reliable analytical separations, featuring 5 µm particles and fully double end‑capped C18 bonding.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) chemistry, this stationary phase:
Uses complete end‑capping to minimize silanol interactions
Improves peak shape for polar and basic analytes
Provides consistent and reproducible chromatographic results
This column is ideal as a USP L1 alternate and as a dependable platform for routine and method development workflows.
100 mm Length – Increased Resolution
Higher resolving power compared to 50 mm columns
Improved separation of closely related compounds
Increased peak capacity for complex samples
Ideal when greater resolution is required without extended run times
3.0 mm ID – Solvent‑Saving Analytical Performance
Reduces mobile phase consumption vs 4.6 mm columns
Requires moderate flow rates with improved sensitivity vs 4.6 mm ID
Typical flow rate range: ~0.4–0.8 mL/min
Optimal starting range: ~0.5–0.7 mL/min
✅ Ideal for solvent savings and improved detection sensitivity ⚠️ Requires a system capable of stable, consistent reduced flow rates
Flow Rate & System Requirements (3.0 mm ID, 5 µm)
✅ Operates under standard high‑pressure HPLC conditions ✅ Produces lower backpressure than 3 µm and 1.8 µm columns, while remaining a high‑pressure column
Requires:
HPLC system capable of stable mid‑to‑low flow operation
Properly sized detector flow cell for reduced internal volume systems
⚠️ Systems must maintain accurate flow control at reduced flow rates for optimal performance.
Why Choose RP C18 (5 µm)
High‑Pressure Robustness & Reliability
Designed for standard high‑pressure HPLC systems
Lower backpressure relative to smaller particle sizes
Ideal for rugged, long‑lifetime analytical methods
Reliable Hydrophobic Retention
Strong reversed‑phase retention for hydrophobic and neutral compounds
Excellent for long‑chain fatty acids
Provides greater retention than C8 phases
Method Development & USP Compatibility
Ideal for new method development
Suitable for USP L1 methods using 5 µm columns
Reliable alternate or replacement column
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for basic analytes
High Purity Silica
Consistent chromatographic performance
Reduced variability across runs
Durable & Cost‑Efficient
Longer column lifetime vs smaller particle sizes
Reduced system strain
Lower maintenance and replacement frequency
Typical Applications
Pharmaceutical and QC analysis
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Method development and optimization
Routine analytical HPLC workflows
USP L1 reversed‑phase methods
Important Usage Notes
Operates under standard high‑pressure HPLC conditions
Requires systems capable of reliable reduced flow rates (3.0 mm ID)
Ideal for solvent‑saving analytical methods
Best used when balancing resolution, sensitivity, and solvent consumption
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable