Catalog Number - 68518-10P-2-3M

The 68518‑10P‑2‑3M Cogent RP C18 Wide Pore HPLC Column is a robust, reversed‑phase column designed for improved separation of large molecular weight analytes, combining 5 µm particles with a 300 Å wide‑pore structure.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Reliable, reproducible chromatographic performance

The 300 Å pore size allows large molecules to fully access the stationary phase, eliminating restricted diffusion and poor recovery seen with 100 Å columns.

100 mm Length – Increased Resolution for Biomolecules

• Higher resolving power compared to 50 mm columns

• Improved separation of proteins, peptides, and complex biomolecules

• Increased peak capacity for complex mixtures

• Ideal for LC‑MS workflows requiring enhanced resolution

This format is commonly selected when greater separation is needed without moving to longer (150 mm) columns.

Flow Rate & System Requirements (2.1 mm ID, 5 µm, 300 Å)

• Typical flow rate range: ~0.2–0.5 mL/min

• Optimal starting range: ~0.25–0.40 mL/min

• Requires:

  • HPLC system capable of stable low‑flow delivery

  • Low‑volume detector flow cell

  • Precision injection system

✅ Operates under standard high‑pressure HPLC conditions ✅ Produces lower backpressure than 3 µm and 1.8 µm columns, while remaining a high‑pressure column

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Enables efficient separation of:

  • Proteins

  • Peptides

  • Oligonucleotides

• Eliminates mass transfer limitations of 100 Å stationary phases

Improved Recovery & Peak Shape

• Better analyte penetration into the stationary phase

• Improved peak symmetry and reproducibility

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (5 µm) Performance Advantages

High‑Pressure Robustness & Reliability

• Designed for standard high‑pressure HPLC systems

• Lower backpressure relative to smaller particle sizes

• Ideal for rugged, repeatable analytical workflows

Reliable Hydrophobic Retention

• Strong retention for hydrophobic and neutral compounds

• Effective for lipids, fatty acids, and biomolecules

• Provides greater retention than C8 phases

Method Development Flexibility

• Ideal for LC‑MS method development requiring robustness

• Suitable for USP L1 wide‑pore applications

• Flexible across a wide range of analytes

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Better performance for basic and polar analytes

High Purity Silica

• Consistent chromatographic results

• Reduced variability between runs

Durable & Cost‑Efficient

• Longer column lifetime vs smaller particles

• Reduced system strain

• Lower maintenance and replacement frequency

Typical Applications

• LC‑MS biomolecule workflows

• Protein and peptide separations

• Oligonucleotide analysis

• Lipids and hydrophobic analytes

• Long‑chain fatty acids

• Complex mixtures requiring enhanced resolution

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Operates under standard high‑pressure HPLC conditions

• Requires stable low‑flow performance (2.1 mm ID)

• Ideal when robustness and biomolecule compatibility are prioritized over maximum efficiency