HPLC Column, RP C18, 5um, 2.1mm ID x 100mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 68518‑10P‑2 Cogent RP C18 HPLC Column is a durable, reversed‑phase column designed for reliable analytical separations, featuring 5 µm particles and fully double end‑capped C18 bonding.
Manufactured with high‑purity silica and traditional octadecylsilane (C18) chemistry, this stationary phase:
Uses complete end‑capping to minimize silanol interactions
Improves peak shape for polar and basic analytes
Provides consistent, reproducible chromatographic performance
This column is widely used as a USP L1 alternate column and for routine LC‑MS and analytical method development.
100 mm Length – Increased Resolution for LC‑MS
Higher resolving power compared to 50 mm columns
Improved separation of closely related compounds
Increased peak capacity for complex samples
Ideal for LC‑MS workflows requiring enhanced resolution
This format is commonly selected when greater separation is needed while maintaining reasonable run times.
Flow Rate & System Requirements (2.1 mm ID, 5 µm)
Typical flow rate range: ~0.2–0.5 mL/min
Optimal starting range: ~0.25–0.40 mL/min
Requires:
HPLC system capable of stable low‑flow delivery
Low‑volume detector flow cell
Reliable injection system
✅ Operates under standard high‑pressure HPLC conditions ✅ Generates lower backpressure than 3 µm and 1.8 µm columns, while remaining a high‑pressure analytical column
Why Choose RP C18 (5 µm)
High‑Pressure Robustness & Reliability
Designed for standard HPLC high‑pressure operation
Lower backpressure relative to smaller particle columns
Ideal for rugged, long‑lifetime analytical methods
Reliable Hydrophobic Retention
Strong retention for hydrophobic and neutral compounds
Excellent for long‑chain fatty acids
Provides longer retention than C8 phases
Method Development & USP Compatibility
Ideal as a starting point for new methods
Suitable for USP L1 methods using 5 µm particles
Frequently used in LC‑MS where robustness is prioritized
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for basic analytes
High Purity Silica
Consistent chromatography
Excellent reproducibility
Durable & Cost‑Efficient
Longer column lifetime vs smaller particle sizes
Reduced system strain
Lower maintenance and replacement frequency
Typical Applications
LC‑MS analytical workflows
Pharmaceutical and QC analysis
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Method development and optimization
USP L1 reversed‑phase methods
Important Usage Notes
Operates under standard high‑pressure HPLC conditions
Requires stable low‑flow system capability (2.1 mm ID)
Ideal for applications requiring increased resolution without UHPLC pressure demands
Preferred when robustness and reproducibility are more important than maximum efficiency