Catalog Number - 68518-10P

The 68518‑10P Cogent RP C18 HPLC Column is a durable, reversed‑phase column engineered for reliable analytical performance, utilizing 5 µm particles and fully double end‑capped C18 bonding to deliver consistent retention, improved peak shape, and long column lifetime.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) chemistry, this stationary phase:

• Uses complete end‑capping to minimize silanol interactions

• Improves peak shape for polar and basic analytes

• Provides reproducible and robust chromatography

This column is ideal as a USP L1 alternate and a dependable choice for routine analytical methods and method development.

100 mm Length – Increased Resolution

• Higher resolving power than 50 mm columns

• Improved separation of closely related compounds

• Increased peak capacity for complex mixtures

• Ideal when shorter columns do not provide enough resolution

This configuration is commonly used when faster methods are needed but added resolution is required beyond a 50 mm column.

4.6 mm ID – Standard Analytical Performance

• Industry‑standard HPLC column dimension

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong detector response

• Excellent reproducibility

• Easy method transfer across systems

Flow Rate & System Requirements (4.6 mm ID, 5 µm)

✅ Operates under standard high‑pressure HPLC conditions ✅ Generates lower backpressure than 3 µm and 1.8 µm columns, while remaining a high‑pressure analytical column

• Suitable for conventional HPLC systems

• Tolerant of:

  • Higher flow rates

  • Viscous mobile phases

  • Extended analytical runs

Why Choose RP C18 (5 µm)

High‑Pressure Robustness & Reliability

• Designed for standard high‑pressure HPLC systems

• Lower backpressure relative to smaller particle columns

• Ideal for rugged, long‑lifetime analytical workflows

Reliable Hydrophobic Retention

• Strong retention for hydrophobic and neutral compounds

• Excellent for long‑chain fatty acids

• Provides greater retention than C8 phases

Method Development & USP Compatibility

• Ideal for new method development

• Suitable for USP L1 methods using 5 µm particles

• Reliable alternate or replacement column

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Better performance for basic analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability across runs

Durable & Cost‑Efficient

• Longer column lifetime vs smaller particle sizes

• Reduced system strain

• Lower maintenance and replacement frequency

Typical Applications

• Pharmaceutical and QC analysis

• Hydrophobic and neutral compound separations

• Long‑chain fatty acids

• Method development and optimization

• Routine analytical HPLC workflows

• USP L1 reversed‑phase methods

Important Usage Notes

• Operates under standard high‑pressure HPLC conditions

• Compatible with conventional analytical systems

• Ideal for applications requiring improved resolution with maximum robustness

• Preferred when reliability and system compatibility outweigh maximum efficiency