CUSTOM ITEM: HPLC Column, RP C18, Wide Pore, 5um, 4.0mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.
Additional Info:
The 68518‑05P‑4‑3M Cogent RP C18 Wide Pore HPLC Column is a robust, reversed‑phase column designed for large molecular weight analytes, combining 5 µm particles with a 300 Å wide‑pore structure to deliver reliable retention, improved recovery, and reproducible performance.
Manufactured using high‑purity silica and traditional octadecylsilane (C18) bonding, and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Consistent and durable chromatographic performance
The 300 Å pore size allows large molecules to fully penetrate the stationary phase, preventing restricted diffusion and loss of efficiency seen with standard 100 Å columns.
50 mm Length – Fast Biomolecule Analysis
Designed for short run times and rapid separations
Lower backpressure compared to longer columns
Ideal for high‑throughput screening and method development
Suitable for fast biomolecule and LC‑MS workflows
4.0 mm ID – Solvent‑Saving Analytical Performance
Comparable chromatographic behavior to 4.6 mm ID columns
Reduces mobile phase consumption and waste
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.9–1.1 mL/min
Benefits:
Lower operating cost
Reduced solvent usage
Compatible with standard HPLC systems
Flow Rate & System Requirements (4.0 mm ID, 5 µm, 300 Å)
Typical flow rate range: ~0.8–1.5 mL/min
Optimal starting range: ~0.9–1.2 mL/min
✅ Operates under standard high‑pressure HPLC conditions ✅ Produces lower backpressure than 3 µm and 1.8 µm columns, but remains a high‑pressure analytical column
Why Choose Wide Pore (300 Å) C18
Designed for Large Molecules
Enables separation of:
Proteins
Peptides
Oligonucleotides
Eliminates mass transfer limitations of 100 Å phases
Improved Recovery & Peak Shape
Better analyte interaction and penetration
Improved peak symmetry and reproducibility
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
RP C18 (5 µm) Performance Advantages
High‑Pressure Robustness & Reliability
Designed for standard high‑pressure HPLC systems
Lower backpressure relative to smaller particle columns
Ideal for rugged, long‑lifetime analytical methods
Reliable Hydrophobic Retention
Strong retention for hydrophobic and neutral compounds
Effective for lipids, fatty acids, and biomolecules
Provides greater retention than C8 phases
Method Development Versatility
Ideal for screening and early‑stage method development
Suitable for USP L1 wide‑pore applications
Flexible and reproducible platform
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for polar and basic analytes
High Purity Silica
Consistent chromatographic results
Reduced variability between runs
Durable & Cost‑Efficient
Longer column lifetime vs smaller particles
Reduced system strain
Lower maintenance requirements
Typical Applications
Protein and peptide separations
Oligonucleotide analysis
Biomolecule HPLC and LC‑MS workflows
Lipids and hydrophobic analytes
Long‑chain fatty acids
Wide‑pore reversed‑phase analytical methods
Important Usage Notes
Designed for large molecule separations using 300 Å pore media
Operates under standard high‑pressure HPLC conditions
Compatible with conventional HPLC systems
Ideal when robustness and biomolecule compatibility are prioritized over maximum efficiency
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable