Catalog Number - 68518-05P-3M

The 68518‑05P‑3M Cogent RP C18 Wide Pore HPLC Column is a robust, reversed‑phase column optimized for large molecular weight analytes, combining 5 µm particles with a 300 Å wide‑pore structure to deliver reliable retention, improved recovery, and consistent chromatographic performance.

Manufactured with high‑purity silica and traditional octadecylsilane (C18) bonding, and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• High reproducibility and durability

The 300 Å pore size allows large molecules to fully access the stationary phase, eliminating restricted diffusion and poor recovery commonly seen with 100 Å columns.

50 mm Length – Fast Biomolecule Screening

• Designed for rapid separations and short run times

• Lower backpressure compared to longer columns

• Ideal for high‑throughput screening (HTS)

• Very popular for fast biomolecule method development and screening workflows

4.6 mm ID – Standard Analytical Performance

• Industry‑standard HPLC column format

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong detector response

• Robust reproducibility

• Easy method transfer between laboratories

Flow Rate & System Requirements (4.6 mm ID, 5 µm, 300 Å)

✅ Operates under standard high‑pressure HPLC conditions ✅ Provides lower backpressure than 3 µm and 1.8 µm columns, while remaining a high‑pressure analytical column

• Suitable for conventional HPLC systems

• Tolerant of:

  • Higher flow rates

  • Viscous mobile phases

  • Routine analytical throughput demands

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Enables separation of:

  • Proteins

  • Peptides

  • Oligonucleotides

• Eliminates mass transfer limitations of 100 Å stationary phases

Improved Recovery & Peak Shape

• Better analyte penetration into stationary phase

• Improved peak symmetry and reproducibility for biomolecules

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (5 µm) Performance Advantages

High‑Pressure Robustness & Reliability

• Designed for standard high‑pressure HPLC systems

• Lower backpressure relative to smaller particle columns

• Ideal for rugged, repeatable analytical workflows

Reliable Hydrophobic Retention

• Strong retention for hydrophobic and neutral compounds

• Effective for lipids, fatty acids, proteins, and peptides

• Provides greater retention than C8 phases

High‑Throughput Capability

• Ideal for screening and method development workflows

• Popular in high‑throughput environments

• Flexible across many analytical applications

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Better performance for polar and basic analytes

High Purity Silica

• Consistent chromatographic results

• Reduced variability between runs

Durable & Cost‑Efficient

• Longer column lifetime vs smaller particle sizes

• Reduced system strain

• Lower maintenance requirements

Typical Applications

• High‑throughput screening (HTS)

• Protein and peptide separations

• Oligonucleotide analysis

• Biomolecule HPLC workflows

• Lipids and hydrophobic compounds

• Long‑chain fatty acids

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Operates under standard high‑pressure HPLC conditions

• Compatible with conventional analytical systems

• Ideal for fast screening and high‑throughput biomolecule workflows