Catalog Number - 68518-05P-2-3M

The 68518‑05P‑2‑3M Cogent RP C18 Wide Pore HPLC Column is a robust, reversed‑phase column designed for large molecular weight analytes, combining 5 µm particles with a 300 Å wide‑pore structure to deliver reliable retention, improved recovery, and consistent performance.

Manufactured with high‑purity silica and traditional octadecylsilane (C18) bonding, and fully double end‑capped, this stationary phase provides:

• Reduced silanol activity

• Improved peak shape for proteins, peptides, and large polar analytes

• Excellent reproducibility and durability

The 300 Å pore size ensures biomolecules can fully access the stationary phase, eliminating steric exclusion limitations common with 100 Å media.

50 mm Length – Fast Biomolecule Analysis

• Designed for short run times and rapid separations

• Lower backpressure compared to longer columns

• Ideal for high‑throughput screening and method development

• Frequently used in LC‑MS workflows requiring speed and reliability

Flow Rate & System Requirements (2.1 mm ID, 5 µm, 300 Å)

• Typical flow rate range: ~0.2–0.5 mL/min

• Optimal starting range: ~0.25–0.40 mL/min

• Requires:

  • HPLC system capable of stable low‑flow delivery

  • Low‑volume detector flow cell

  • Precision injection system

✅ Operates under standard high‑pressure HPLC conditions ✅ Produces lower backpressure than 3 µm and 1.8 µm columns, while remaining a high‑pressure column

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Enables separation of:

  • Proteins

  • Peptides

  • Oligonucleotides

• Eliminates mass transfer limitations seen in 100 Å columns

Improved Recovery & Peak Shape

• Better analyte interaction and penetration

• Improved peak symmetry and reproducibility

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (5 µm) Performance Advantages

High‑Pressure Robustness & Compatibility

• Designed for standard high‑pressure HPLC systems

• Lower backpressure relative to smaller particles

• Highly tolerant of viscous mobile phases and demanding methods

Reliable Hydrophobic Retention

• Strong retention for hydrophobic and neutral compounds

• Effective for lipids, fatty acids, and biomolecules

• Provides greater retention than C8 phases

Method Development Versatility

• Ideal for screening and early‑stage method development

• Suitable for USP L1 wide‑pore applications

• Flexible and reproducible platform

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Better performance for polar and basic analytes

High Purity Silica

• Consistent chromatographic results

• Reduced run‑to‑run variability

Durable & Cost‑Efficient

• Longer column lifetime vs smaller particles

• Reduced system strain

• Lower maintenance requirements

Typical Applications

• LC‑MS biomolecule workflows

• Protein and peptide separations

• Oligonucleotide analysis

• Lipids and hydrophobic compounds

• Long‑chain fatty acids

• Wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Operates under standard high‑pressure HPLC conditions

• Requires stable low‑flow performance (2.1 mm ID)

• Ideal when robustness and biomolecule compatibility are prioritized over maximum efficiency