HPLC Column, RP C18, 5um, 2.1mm ID x 50mm Length, 100A. Cogent Brand. 1 EA.

Additional Info:

The 68518‑05P‑2 Cogent RP C18 HPLC Column is a robust, reversed‑phase column featuring 5 µm particles and fully double end‑capped C18 bonding, delivering reliable retention, excellent durability, and consistent chromatographic performance.

Manufactured using high‑purity silica and traditional octadecylsilane (C18) bonding, this stationary phase:

  • Uses complete end‑capping to minimize silanol interactions

  • Improves peak shape for polar and basic analytes

  • Provides consistent and reproducible analytical results

This column is ideal as a USP L1 alternate column and a dependable choice for routine analytical methods.

50 mm Length – Fast Analysis & Throughput

  • Designed for rapid separations and short run times

  • Lower backpressure compared to longer columns

  • Ideal for high‑throughput workflows and screening

  • Frequently used in LC‑MS methods requiring fast cycle times

Flow Rate & System Requirements (2.1 mm ID, 5 µm)

  • Typical flow rate range: ~0.2–0.5 mL/min

  • Optimal starting range: ~0.25–0.40 mL/min

  • Requires:

    • HPLC system capable of stable low‑flow delivery

    • Low‑volume detector flow cell

    • Reliable injection system

✅ This column operates under standard HPLC high‑pressure conditions ✅ Produces lower backpressure than 3 µm and 1.8 µm columns, but is still firmly within high‑pressure HPLC operation

Why Choose RP C18 (5 µm)

High‑Pressure Robustness & Reliability

  • Designed for standard high‑pressure HPLC systems

  • Lower backpressure compared to smaller particles

  • Ideal for rugged, long‑term analytical methods

Reliable Hydrophobic Retention

  • Strong reversed‑phase retention for hydrophobic and neutral compounds

  • Excellent for long‑chain fatty acids

  • Provides longer retention than C8 phases

USP Method Compatibility

  • Suitable for USP L1 methods using 5 µm columns

  • Reliable alternate or replacement column

Cogent RP C18™ Advantages

Fully End‑Capped Surface

  • Reduces secondary interactions

  • Improves peak symmetry

  • Enhances performance for basic analytes

High Purity Silica

  • Consistent results across runs

  • Excellent reproducibility

Durable & Cost‑Efficient

  • Longer column lifetime compared to smaller particles

  • Reduced system stress vs higher efficiency media

  • Lower maintenance frequency

Typical Applications

  • LC‑MS analytical workflows

  • Pharmaceutical and QC analysis

  • Hydrophobic and neutral compound separations

  • Long‑chain fatty acids

  • Routine analytical HPLC methods

  • USP L1 reversed‑phase methods

Important Usage Notes

  • Operates under standard high‑pressure HPLC conditions

  • Requires stable low‑flow system performance (2.1 mm ID)

  • Ideal for rugged, repeatable methods

  • Chosen when robustness and system compatibility are prioritized over maximum efficiency

 

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