Catalog Number - 68518-05P

The 68518‑05P Cogent RP C18 HPLC Column is a durable, reversed‑phase column designed for reliable routine analysis and method development, featuring 5 µm particles and fully double end‑capped C18 bonding.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) chemistry, this stationary phase provides:

• Complete end‑capping to minimize silanol interactions

• Improved peak shape for polar and basic analytes

• Consistent and reproducible chromatographic performance

This column is ideal as a USP L1 alternate column and a trusted starting point for new analytical methods.

50 mm Length – Fast Analysis & Method Scouting

• Designed for short run times and rapid separations

• Lower backpressure compared to longer columns

• Ideal for high‑throughput screening (HTS)

• Frequently used as a scouting column for preparative method development

4.6 mm ID – Standard Analytical Performance

• Industry‑standard HPLC column format

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong detector response

• Excellent reproducibility

  Straightforward method transfer across laboratories

Flow Rate & System Requirements (4.6 mm ID, 5 µm)

✅ Operates under standard high‑pressure HPLC conditions ✅ Generates lower backpressure than 3 µm and 1.8 µm columns, but remains a high‑pressure analytical column

• Suitable for conventional HPLC systems

• More tolerant of:

  • Higher flow rates

  • Viscous mobile phases

  • Longer method durations

Why Choose RP C18 (5 µm)

High‑Pressure Robustness & Durability

• Designed for standard HPLC high‑pressure operation

• Lower backpressure relative to smaller particle columns

• Ideal for rugged, long‑lifetime analytical methods

Reliable Hydrophobic Retention

• Strong reversed‑phase retention for hydrophobic and neutral compounds

• Excellent for long‑chain fatty acids

• Provides longer retention than C8 phases

Method Development & USP Compatibility

• Ideal as a starting point for new methods

• Suitable for USP L1 methods using 5 µm particles

• Commonly used for analytical to preparative method scouting

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Better performance for basic analytes

High Purity Silica

• Consistent analytical performance

• Reduced variability between runs and columns

Durable & Cost‑Efficient

• Longer column lifetime vs smaller particles

• Reduced system strain

• Lower maintenance and replacement frequency

Typical Applications

• Pharmaceutical and QC analysis

• Hydrophobic and neutral compound separations

• Long‑chain fatty acids

• Method development and optimization

• Preparative method scouting

• USP L1 reversed‑phase workflows

Important Usage Notes

• Operates under standard high‑pressure HPLC conditions

• Compatible with conventional analytical systems

• Ideal for fast method development and screening workflows

• Frequently used as a starting point before scale‑up to preparative columns