Prep HPLC Column, RP C12, 5um, 100A. 21.2mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C12™ Preparative HPLC Columns are designed for purification, scale-up, and preparative chromatography applications requiring unique selectivity, rapid equilibration, and reproducible retention. The stationary phase is manufactured using a rigid adamantane-shaped carbon silane ligand bonded to ultra-high purity silica and fully double end capped to minimize secondary interactions. This distinctive bonded phase chemistry offers chromatographic behavior between traditional C8 and C18 phases, making it especially useful for preparative separations of complex mixtures and compounds where alternative selectivity is beneficial.
68512-P21-250 is packed with 5 µm RP C12™ particles featuring a 100 Å pore size in a 21.2 mm ID x 250 mm preparative format. The extended column length provides maximum resolving power and purification efficiency while maintaining substantial sample loading capacity for purification, fraction collection, and analytical-to-preparative method scale-up. The RP C12™ phase typically provides less retention than C18 columns but greater retention than C8 columns for many hydrophobic compounds, offering unique selectivity that can improve preparative separations. The stationary phase is also compatible with highly aqueous mobile phases, including 100% water, without phase collapse or loss of retention.
The 21.2 mm ID x 250 mm format is commonly selected when maximum purification performance and chromatographic resolution are required. Typical flow rates are approximately 15-25 mL/min depending on mobile phase composition and system configuration. A specialized preparative HPLC system capable of high flow rates, large injection volumes, and increased sample loading is required for optimum performance.
Key Benefits
RP C12™ stationary phase
Adamantane-shaped bonded ligand
Fully double end capped
Ultra-high purity silica support
100 Å pore size
Unique selectivity profile
Compatible with 100% aqueous mobile phases
Rapid equilibration between runs
High sample loading capacity
Maximum purification efficiency
Typical Applications
Preparative HPLC
Compound purification
Fraction collection
Method scale-up
Pharmaceutical purification
Natural product isolation
Process development
Pilot-scale chromatography
Complex mixture separations
Alternative selectivity applications
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: RP C12™
Chromatography Mode: Reversed Phase
Bonded Phase: Adamantane-Shaped C12 Ligand
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 100 Å
Column Dimensions: 21.2 mm ID x 250 mm
Recommended Flow Rate: 15-25 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Special Order Item Information
Important Notes
Designed for preparative reversed-phase purification
Provides a selectivity profile between traditional C8 and C18 phases
Compatible with highly aqueous and 100% water mobile phases
Rapid equilibration minimizes preparation time between runs
Suitable for hydrophobic and moderately non-polar compounds
21.2 mm ID format provides substantial sample loading capacity
250 mm length provides maximum resolution and purification efficiency
5 µm particles support preparative flow rates with manageable backpressure
Typical operating range is approximately 15-25 mL/min
Requires a specialized preparative HPLC system capable of high flow rates and sample loading
Suitable for analytical-to-preparative method scale-up
This is a Special Order Item