Catalog Number - 68512-P21-100

Cogent RP C12™ Preparative HPLC Columns are designed for purification, scale-up, and preparative chromatography applications requiring unique selectivity, rapid equilibration, and reproducible retention. The stationary phase is manufactured using a rigid adamantane-shaped carbon silane ligand bonded to ultra-high purity silica and fully double end capped to minimize secondary interactions. This distinctive bonded phase chemistry offers chromatographic behavior between traditional C8 and C18 phases, making it especially useful for preparative separations of complex mixtures and compounds where alternative selectivity is beneficial.

68512-P21-100 is packed with 5 µm RP C12™ particles featuring a 100 Å pore size in a 21.2 mm ID x 100 mm preparative format. The large internal diameter provides substantial sample loading capacity for purification, fraction collection, and analytical-to-preparative method scale-up. The RP C12™ phase typically provides less retention than C18 columns but greater retention than C8 columns for many hydrophobic compounds, offering unique selectivity that can improve preparative separations. The stationary phase is also compatible with highly aqueous mobile phases, including 100% water, without phase collapse or loss of retention.

The 21.2 mm ID format is commonly used for preparative purification, process development, and pilot-scale chromatography applications. Typical flow rates are approximately 15-25 mL/min depending on mobile phase composition and system configuration. A specialized preparative HPLC system capable of high flow rates, large injection volumes, and increased sample loading is required for optimum performance.

Key Benefits

• RP C12™ stationary phase

• Adamantane-shaped bonded ligand

• Fully double end capped

• Ultra-high purity silica support

• 100 Å pore size

• Unique selectivity profile

• Compatible with 100% aqueous mobile phases

• Rapid equilibration between runs

• High sample loading capacity

• Ideal for preparative purification

Typical Applications

• Preparative HPLC

• Compound purification

• Fraction collection

• Method scale-up

• Pharmaceutical purification

• Natural product isolation

• Process development

• Pilot-scale chromatography

• Complex mixture separations

• Alternative selectivity applications

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: RP C12™

• Chromatography Mode: Reversed Phase

• Bonded Phase: Adamantane-Shaped C12 Ligand

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 21.2 mm ID x 100 mm

• Recommended Flow Rate: 15-25 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Special Order Item Information

• Please contact Customer Service for formal quotation, lead time, and ordering assistance.

Important Notes

• Designed for preparative reversed-phase purification

• Provides a selectivity profile between traditional C8 and C18 phases

• Compatible with highly aqueous and 100% water mobile phases

• Rapid equilibration minimizes preparation time between runs

• Suitable for hydrophobic and moderately non-polar compounds

• 21.2 mm ID format provides substantial sample loading capacity

• 100 mm length offers an excellent balance of throughput and resolution

• 5 µm particles support preparative flow rates with manageable backpressure

• Typical operating range is approximately 15-25 mL/min

• Requires a specialized preparative HPLC system capable of high flow rates and sample loading

• Suitable for analytical-to-preparative method scale-up

• This is a Special Order Item

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