HPLC Column, RP C12, 5um, 4.6mm ID x 250mm Length, 100A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C12™ HPLC Columns are designed for reversed-phase separations requiring unique selectivity, rapid equilibration, and reproducible retention. The stationary phase is manufactured using a rigid adamantane-shaped carbon silane ligand bonded to ultra-high purity silica and fully double end capped to minimize secondary interactions. This distinctive bonded phase chemistry offers chromatographic behavior between traditional C8 and C18 phases, making it especially useful for complex mixtures, analytical method development, and high-throughput screening applications.
68512-25P is packed with 5 µm RP C12™ particles featuring a 100 Å pore size in a 4.6 mm ID x 250 mm format. The extended column length provides maximum resolving power and peak capacity, making it ideal for challenging separations that require enhanced resolution. The RP C12™ phase typically provides less retention than C18 columns but greater retention than C8 columns for many hydrophobic compounds, offering an alternative selectivity profile that can improve difficult chromatographic separations. The stationary phase is also compatible with highly aqueous mobile phases, including 100% water, without phase collapse or loss of retention.
The 4.6 mm ID x 250 mm format is one of the most popular configurations for analytical HPLC when maximum resolution is required. It is commonly used for pharmaceutical analysis, complex sample matrices, method development, quality control testing, and research applications. Typical flow rates are approximately 0.8-1.5 mL/min depending on method requirements and mobile phase conditions.
Key Benefits
RP C12™ stationary phase
Adamantane-shaped bonded ligand
Fully double end capped
Ultra-high purity silica support
100 Å pore size
Unique selectivity profile
Compatible with 100% aqueous mobile phases
Rapid column equilibration
Maximum chromatographic resolution
Ideal for complex separations
Typical Applications
Reversed-phase HPLC
Pharmaceutical analysis
Method development
Quality control testing
Complex mixture separations
High-throughput screening (HTS)
Research and development
Hydrophobic compound analysis
Stability studies
Routine analytical HPLC
Specifications
Product Type: HPLC Column
Stationary Phase: RP C12™
Chromatography Mode: Reversed Phase
Bonded Phase: Adamantane-Shaped C12 Ligand
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 100 Å
Column Dimensions: 4.6 mm ID x 250 mm
Recommended Flow Rate: 0.8-1.5 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Important Notes
Provides a selectivity profile between traditional C8 and C18 phases
Compatible with highly aqueous and 100% water mobile phases
Rapid equilibration minimizes time between injections
250 mm length provides maximum resolution and peak capacity
Excellent choice for complex mixtures and challenging separations
Useful for method development requiring alternative selectivity
Suitable for routine analytical and quality control applications
Popular for pharmaceutical analysis and research laboratories
4.6 mm ID format is compatible with most standard HPLC systems
One of the most popular Cogent RP C12™ column configurations