HPLC Column, RP C12, 5um, 2.1mm ID x 100mm Length, 100A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C12™ HPLC Columns are designed for reversed-phase separations requiring unique selectivity, rapid equilibration, and reproducible retention. The stationary phase is manufactured using a rigid adamantane-shaped carbon silane ligand bonded to ultra-high purity silica and fully double end capped to minimize secondary interactions. This distinctive bonded phase chemistry offers chromatographic behavior between traditional C8 and C18 phases, making it especially useful for complex mixtures, LC-MS methods, and analytical method development.
68512-10P-2 is packed with 5 µm RP C12™ particles featuring a 100 Å pore size in a 2.1 mm ID x 100 mm format. The narrow internal diameter provides reduced solvent consumption and excellent compatibility with LC-MS systems, while the 100 mm length offers increased resolving power compared to shorter columns. The RP C12™ phase typically provides less retention than C18 columns but greater retention than C8 columns for many hydrophobic compounds, offering an alternative selectivity profile that can improve challenging separations. The stationary phase is also compatible with highly aqueous mobile phases, including 100% water, without phase collapse or loss of retention.
The 2.1 mm ID x 100 mm format is frequently selected for LC-MS applications, method development, pharmaceutical analysis, and complex sample separations. Typical flow rates are approximately 0.2-0.3 mL/min. Standard HPLC systems used with 2.1 mm ID columns must be capable of delivering stable and reproducible low flow rates, and detector flow cells should be optimized for low-volume chromatography to achieve maximum performance.
Key Benefits
RP C12™ stationary phase
Adamantane-shaped bonded ligand
Fully double end capped
Ultra-high purity silica support
100 Å pore size
Unique selectivity profile
Compatible with 100% aqueous mobile phases
Rapid column equilibration
Excellent for LC-MS applications
Low solvent consumption
Typical Applications
LC-MS methods
LC-MS/MS analysis
Reversed-phase HPLC
High-throughput screening (HTS)
Method development
Pharmaceutical analysis
Complex mixture separations
Quality control testing
Research and development
Hydrophobic compound analysis
Specifications
Product Type: HPLC Column
Stationary Phase: RP C12™
Chromatography Mode: Reversed Phase
Bonded Phase: Adamantane-Shaped C12 Ligand
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 100 Å
Column Dimensions: 2.1 mm ID x 100 mm
Recommended Flow Rate: 0.2-0.3 mL/min
Column Hardware: Stainless Steel
Brand: Cogent
Important Notes
Provides a selectivity profile between traditional C8 and C18 phases
Compatible with highly aqueous and 100% water mobile phases
Rapid equilibration minimizes time between injections
100 mm length provides improved resolution and peak capacity
Excellent choice for LC-MS and LC-MS/MS methods
Useful for complex mixtures requiring alternative selectivity
2.1 mm ID format minimizes solvent consumption and improves MS compatibility
Requires an HPLC system capable of reliable low-flow operation at approximately 0.2-0.3 mL/min
Best performance is achieved using low-volume detector flow cells and LC-MS interfaces