Catalog Number - 68508-25P-3M

Cogent RP C8™ Wide Pore HPLC Columns are designed for reversed-phase separations of larger molecules that require enhanced pore accessibility and efficient mass transfer. The stationary phase consists of a traditional octyl (C8) bonded ligand attached to ultra-high purity silica and is fully double end capped to minimize secondary interactions while providing reproducible retention, excellent peak shape, and long column life. The 300 Å wide pore structure allows proteins, peptides, polysaccharides, and other higher molecular weight compounds to access more of the bonded surface area, making this phase particularly suitable for biomolecule analyses and demanding large-molecule separations.

68508-25P-3M is packed with 5 µm RP C8™ Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 250 mm length format. The 250 mm column length provides maximum resolving power and increased peak capacity for complex separations where the highest analytical performance is required. The wide pore architecture improves accessibility and mass transfer for proteins, peptides, polysaccharides, and long-chain fatty acids that may be partially excluded from conventional 100 Å media. The RP C8™ chemistry provides lower hydrophobic retention than comparable C18 phases while maintaining excellent selectivity for hydrophobic and moderately non-polar compounds.

This column is frequently selected for the analysis of macromolecules using HPLC systems equipped with UV or ELSD detection. Operating efficiently at approximately 1.0-2.0 mL/min, the 4.6 mm ID format is fully compatible with conventional analytical HPLC systems. The combination of wide pore accessibility, high resolving power, and robust performance makes this column an excellent choice for pharmaceutical, biopharmaceutical, and biomolecule characterization applications.

Key Benefits

• RP C8™ Wide Pore stationary phase

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 300 Å wide pore design

• Ideal for proteins, peptides, and polysaccharides

• Maximum analytical resolution

• Increased peak capacity

• Lower retention than C18 phases

• Compatible with UV and ELSD detection

Typical Applications

• Reversed-phase HPLC

• Protein analysis

• Peptide analysis

• Polysaccharide analysis

• Biomolecule separations

• Long-chain fatty acid analysis

• Pharmaceutical analysis

• Biopharmaceutical analysis

• Method development

• High-resolution analytical separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Recommended Flow Rate: 1.0-2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Designed for large molecule reversed-phase separations

• 300 Å pores enhance accessibility for proteins, peptides, polysaccharides, and other biomolecules

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Excellent choice for protein, peptide, polysaccharide, and long-chain fatty acid analyses

• 250 mm length provides maximum resolving power and peak capacity

• 5 µm particles provide low backpressure and broad instrument compatibility

• Compatible with standard HPLC systems operating at conventional analytical flow rates

• Frequently selected for UV and ELSD-based biomolecule analysis

• Manufactured using ultra-high purity silica and fully double end capped bonding technology