Catalog Number - 68508-25P

Cogent RP C8™ HPLC Columns are designed for reliable reversed-phase chromatography using a traditional octyl (C8) bonded stationary phase. Manufactured with ultra-high purity silica and fully double end capped bonding technology, these columns provide reproducible retention, excellent peak shape, and long column life. The RP C8™ phase is a USP L7 equivalent chemistry and is commonly used as an alternative for methods developed on similar C8 phases, including many established USP methods. Compared to C18 columns, RP C8™ generally provides lower hydrophobic retention while maintaining excellent selectivity for many neutral and non-polar compounds.

68508-25P is packed with 5 µm RP C8™ particles featuring a 100 Å pore size in a 4.6 mm ID x 250 mm length format. The 250 mm column length provides maximum resolving power and increased peak capacity for complex separations where the highest analytical performance is required. The RP C8™ stationary phase is especially well suited for hydrophobic compounds, neutral analytes, and long-chain fatty acids while providing lower retention than comparable C18 phases. The 5 µm particle size offers broad compatibility with conventional HPLC systems while maintaining relatively low backpressure.

The 250 mm length format is frequently selected for established analytical methods, method validation, USP method replacement, and applications where shorter columns do not provide sufficient resolution. Operating efficiently at approximately 1.0-2.0 mL/min, this configuration is a popular choice for pharmaceutical, environmental, food, and quality control laboratories requiring robust and reproducible chromatographic performance.

Key Benefits

• RP C8™ stationary phase

• USP L7 equivalent chemistry

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 100 Å pore size

• Maximum analytical resolution

• Increased peak capacity

• Lower retention than C18 phases

• Low backpressure with 5 µm particles

Typical Applications

• Reversed-phase HPLC

• USP L7 method substitution

• Method validation

• Method development

• Pharmaceutical analysis

• Long-chain fatty acid analysis

• Hydrophobic compound separations

• Neutral compound analysis

• Environmental testing

• Quality control testing

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8™

• USP Classification: L7

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Recommended Flow Rate: 1.0-2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Designed for high-resolution reversed-phase HPLC separations

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Excellent choice for long-chain fatty acid separations

• Frequently selected for USP L7 method replacement and transfer

• 250 mm length provides maximum resolving power and peak capacity

• 5 µm particles provide low backpressure and broad instrument compatibility

• Compatible with standard HPLC systems operating at conventional analytical flow rates

• Ideal when complex samples require the highest chromatographic resolution

• Manufactured using ultra-high purity silica and fully double end capped bonding technology