CUSTOM ITEM: HPLC Column, RP C18, Wide Pore, 3um, 4.0mm ID x 250mm Length, 300A. Cogent Brand. 1 EA.
Additional Info:
The 68318‑25P‑4‑3M Cogent RP C18 Wide Pore HPLC Column is designed for maximum separation performance of large molecular weight analytes, combining 3 µm particle size with a 300 Å wide‑pore structure to deliver superior access, retention, and peak shape for biomolecules.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak symmetry for proteins, peptides, and high‑MW analytes
Excellent reproducibility and robustness
The 300 Å pore size allows large molecules to fully access the stationary phase, eliminating restricted diffusion and loss of resolution seen with standard 100 Å columns.
250 mm Length – Maximum Separation & Peak Capacity
Highest resolving power in the 4.0 mm wide‑pore column range
Maximizes peak capacity for highly complex biomolecule mixtures
Ideal for closely related proteins, peptides, and macromolecules
Best suited for final method development and highest purity separations
Provides longer retention for large and difficult analytes
This column is selected when maximum separation is required and shorter columns cannot achieve adequate resolution.
4.0 mm ID – Solvent‑Saving Analytical Performance
Comparable chromatographic performance to 4.6 mm ID columns
Uses less mobile phase, reducing operational cost and waste
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.85–1.10 mL/min
Benefits:
Reduced solvent consumption
Lower operating cost
Compatible with standard HPLC systems
Why Choose Wide Pore (300 Å) C18
Designed for Large Molecules
Enables efficient separation of:
Proteins
Peptides
Oligonucleotides
Eliminates mass transfer limitations of 100 Å phases
Improved Recovery & Peak Shape
Better analyte penetration into stationary phase
Enhanced peak symmetry and reproducibility
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
RP C18 (3 µm) Performance Advantages
Balanced Efficiency & Pressure
Higher efficiency than 5 µm columns
Lower backpressure than sub‑2 µm UHPLC columns
Ideal for standard HPLC systems
USP Method Compatibility
Suitable for USP L1 methods adapted for large molecules
Excellent alternate column for wide‑pore applications
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for polar and basic analytes
High Purity Silica
Consistent chromatography
Reduced variability and improved reproducibility
Cost‑Efficient Operation
Lower pressure vs UHPLC columns
Reduced solvent usage vs larger ID formats
Lower cost per analysis
Typical Applications
Protein and peptide separations
Oligonucleotide analysis
Biomolecule HPLC and LC‑MS workflows
Lipids and hydrophobic compounds
Long‑chain fatty acids
Complex mixtures requiring maximum resolution and purity
Important Usage Notes
Designed for large molecule separations using 300 Å pore media
Compatible with standard HPLC systems
Optimize temperature and mobile phase for biomolecule performance
Ideal when standard 100 Å columns cannot provide sufficient access or resolution
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable