CUSTOM ITEM: HPLC Column, RP C18, 3um, 4.0mm ID x 250mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 68318‑25P‑4 Cogent RP C18 HPLC Column is optimized for maximum separation performance, utilizing 3 µm particles and fully double end‑capped C18 bonding to deliver high efficiency, reproducibility, and strong retention.
Manufactured with high‑purity silica and traditional octadecylsilane (C18) bonding, this stationary phase:
Uses complete end‑capping to minimize silanol interactions
Improves peak shape for polar and basic analytes
Provides robust, reproducible chromatography across applications
This column is widely used as a primary analytical column, as a starting point for new method development, and as a trusted alternate for USP L1 methods using 3 µm phases.
250 mm Length – Maximum Separation & Retention
Highest resolving power in the 4.0 mm RP C18 column range
Maximizes peak capacity for highly complex mixtures
Ideal for closely related compounds and challenging separations
Provides longer retention for difficult analytes
Best suited for final method optimization and high‑resolution analysis
This column is selected when shorter columns (50 or 100 mm) do not provide sufficient separation.
4.0 mm ID – Solvent‑Saving Analytical Performance
Provides performance similar to 4.6 mm ID analytical columns
Uses less mobile phase, reducing cost and waste
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.85–1.10 mL/min
Benefits:
Lower solvent consumption
Reduced operating cost
Compatible with standard HPLC systems
Why Choose RP C18 (3 µm)
Balanced Efficiency & Pressure
Higher efficiency than 5 µm columns
Lower backpressure than sub‑2 µm UHPLC columns
Ideal balance between performance and instrument compatibility
Reliable Hydrophobic Retention
Strong retention for hydrophobic and neutral compounds
Excellent for long‑chain fatty acids and similar analytes
Method Development & USP Compatibility
Ideal for new method development
Suitable as a USP L1 alternate column
Flexible starting point for method optimization
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for basic compounds
High Purity Silica
Consistent chromatography
Reduced variability between runs and columns
Cost‑Efficient Operation
Lower pressure requirements vs UHPLC columns
Reduced solvent usage vs larger ID columns
Lower cost per analysis
Typical Applications
Pharmaceutical and QC analysis
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Complex multi‑component mixtures
Method development and validation
USP L1 reversed‑phase methods
Important Usage Notes
Compatible with standard HPLC systems
Monitor pressure for long columns and viscous mobile phases
Ideal for maximum‑resolution analytical workflows
Best suited when maximum separation and retention are required
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable