Catalog Number - 68318-25P-3M

The 68318‑25P‑3M Cogent RP C18 Wide Pore HPLC Column is engineered for maximum separation performance of large molecular weight analytes, utilizing 3 µm particles and a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, peak capacity, and reproducibility.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, and fully double end‑capped, this stationary phase offers:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible chromatographic performance

The 300 Å pore size enables large molecules to fully access the stationary phase, preventing restricted diffusion and poor recovery associated with standard 100 Å columns.

250 mm Length – Maximum Separation & Peak Capacity

• Highest resolving power in the 4.6 mm wide‑pore C18 range

• Maximizes peak capacity for highly complex biomolecule mixtures

• Ideal for closely related proteins, peptides, and macromolecules

• Provides extended retention for large and difficult analytes

• Best suited for final method optimization and highest purity separations

This column is selected when shorter formats (50 mm or 100 mm) cannot achieve required separation or resolution.

4.6 mm ID – Standard Analytical Performance

• Industry‑standard HPLC column dimension

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong detector response

• Excellent reproducibility

• Easy method transfer across instruments and labs

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Enables efficient separation of:

  • Proteins

  • Peptides

  • Oligonucleotides

• Eliminates mass transfer limitations seen in 100 Å columns

Improved Recovery & Peak Shape

• Greater analyte penetration into stationary phase

• Improved peak symmetry and reproducibility

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (3 µm) Performance Advantages

Balanced Efficiency & Pressure

• Higher efficiency than 5 µm columns

• Lower backpressure than sub‑2 µm UHPLC columns

• Ideal for standard HPLC systems

USP Method Compatibility

• Suitable for USP L1 methods for large molecule applications

• Excellent alternate column for wide‑pore separations

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for polar and basic analytes

High Purity Silica

• Consistent chromatography

• Reduced variability between runs

Cost‑Efficient Operation

• Lower pressure requirements vs UHPLC columns

• Reduced solvent waste compared to multiple shorter runs

• Lower cost per analysis

Typical Applications

• Protein and peptide separations

• Oligonucleotide analysis

• Biomolecule HPLC and LC‑MS workflows

• Lipids and hydrophobic compounds

• Long‑chain fatty acids

• Complex mixtures requiring maximum separation and purity

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Compatible with standard HPLC systems

• Optimize temperature and mobile phase to maximize biomolecule separation performance

• Ideal when standard 100 Å columns cannot provide sufficient analyte access or resolution