CUSTOM ITEM: HPLC Column, RP C18, Wide Pore, 3um, 4.0mm ID x 150mm Length, 300A. Cogent Brand. 1 EA.
Additional Info:
The 68318‑15P‑4‑3M Cogent RP C18 Wide Pore HPLC Column is engineered for maximum‑resolution separations of large molecular weight analytes, combining 3 µm particle size with a 300 Å wide‑pore structure to deliver enhanced access, retention, and peak shape for biomolecular compounds.
Manufactured using high‑purity silica and traditional octadecylsilane (C18) bonding, and fully double end‑capped, this stationary phase provides:
Reduced silanol activity
Improved peak symmetry for proteins, peptides, and large polar analytes
Excellent reproducibility and robustness
The 300 Å pore size ensures large molecules can fully interact with the stationary phase, preventing restricted diffusion and poor recovery often seen with standard 100 Å columns.
150 mm Length – Maximum Resolution & Peak Capacity
Highest resolving power in the 4.0 mm wide‑pore column range
Maximizes peak capacity for complex biomolecule mixtures
Ideal for closely related proteins, peptides, and macromolecules
Best suited for final method optimization and high‑purity separations
Longer run times compared to 50 mm and 100 mm formats
4.0 mm ID – Solvent‑Saving Analytical Performance
Provides chromatography similar to 4.6 mm ID columns
Uses less mobile phase and reduces solvent cost
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.85–1.10 mL/min
Benefits:
Lower operating cost
Reduced solvent waste
Fully compatible with standard HPLC systems
Why Choose Wide Pore (300 Å) C18
Designed for Large Molecules
Enables access for:
Proteins
Peptides
Oligonucleotides
Eliminates mass transfer limitations of 100 Å columns
Improved Recovery & Peak Shape
Better analyte interaction with stationary phase
Improved peak symmetry and reproducibility
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
RP C18 (3 µm) Performance Advantages
Balanced Efficiency & Pressure
Higher efficiency than 5 µm columns
Lower backpressure than 1.8 µm UHPLC columns
Ideal for standard HPLC systems
USP Method Compatibility
Suitable for USP L1 methods adapted for large molecule separations
Excellent alternate column for wide‑pore applications
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Better performance for polar and basic analytes
High Purity Silica
Consistent chromatography
Reduced run‑to‑run variability
Cost‑Efficient Operation
Lower pressure requirements vs UHPLC columns
Reduced solvent consumption vs larger ID formats
Lower cost per analysis
Typical Applications
Protein and peptide separations
Oligonucleotide analysis
Biomolecule HPLC and LC‑MS workflows
Lipids and hydrophobic compounds
Long‑chain fatty acids
Complex mixtures requiring maximum resolution
Important Usage Notes
Designed for large molecule separations using 300 Å pore media
Compatible with standard HPLC systems
Optimize temperature and mobile phase to maximize biomolecule performance
Ideal when 100 Å columns limit analyte access or resolution
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable