Catalog Number - 68318-15P-3M

The 68318‑15P‑3M Cogent RP C18 Wide Pore HPLC Column is optimized for maximum‑resolution separations of large molecular weight analytes, combining 3 µm particles with a 300 Å pore structure to provide enhanced analyte accessibility, superior peak shape, and high reproducibility.

This stationary phase is manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and is fully double end‑capped, offering:

• Reduced silanol interactions

• Improved peak symmetry for proteins, peptides, and large polar analytes

• Robust, reproducible chromatography

The 300 Å wide‑pore design enables large molecules to fully interact with the stationary phase, eliminating restricted diffusion and poor recovery associated with 100 Å columns.

150 mm Length – Maximum Resolution & Peak Capacity

• Highest resolving power in the 4.6 mm wide‑pore C18 range

• Maximizes peak capacity for complex biomolecule mixtures

• Ideal for closely related proteins, peptides, and macromolecules

• Preferred for analytical workflows requiring highest purity separations

• Longer run times compared to 50 mm and 100 mm formats

This configuration is ideal when maximum separation performance is required over speed.

4.6 mm ID – Standard Analytical Performance

• Industry‑standard HPLC column format

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong detector response

• Reliable reproducibility

  Simple method transfer across systems

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Enables separation of:

  • Proteins

  • Peptides

  • Oligonucleotides

• Eliminates mass transfer limitations of 100 Å stationary phases

Improved Recovery & Peak Shape

• Better analyte penetration into stationary phase

• Enhanced peak symmetry and reproducibility for biomolecules

Maintains Classic C18 Selectivity

• Retains hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (3 µm) Performance Advantages

Balanced Efficiency & Pressure

• Higher efficiency than 5 µm columns

• Lower backpressure than 1.8 µm UHPLC columns

• Ideal for standard HPLC systems

USP Method Compatibility

• Suitable for USP L1 methods adapted for large molecule analysis

• Reliable alternate column for wide‑pore applications

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Better performance for polar and basic analytes

High Purity Silica

• Consistent chromatography

• Reduced variability between runs

Cost‑Efficient Operation

• Lower pressure requirements vs UHPLC systems

• Reduced solvent waste compared to longer methods

• Lower cost per analysis

Typical Applications

• Protein and peptide separations

• Oligonucleotide analysis

• Biomolecule HPLC and LC‑MS workflows

• Lipids and hydrophobic compounds

• Long‑chain fatty acids

  Complex mixtures requiring maximum resolution

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Compatible with standard HPLC systems

• Optimize temperature and mobile phase conditions for biomolecule performance

• Ideal when 100 Å columns limit analyte access or resolution