Catalog Number - 68318-15P-2-3M

The 68318‑15P‑2‑3M Cogent RP C18 Wide Pore HPLC Column is designed for maximum‑resolution separations of large molecular weight analytes, combining 3 µm particles with a 300 Å wide‑pore structure to deliver superior access, retention, and peak shape for biomolecules.

Manufactured using high‑purity silica and traditional octadecylsilane (C18) bonding, and fully double end‑capped, this phase provides:

• Reduced silanol interactions

• Improved peak symmetry for proteins, peptides, and large polar analytes

• Excellent reproducibility and robustness

The 300 Å pore size enables large molecules to fully access the stationary phase, eliminating steric hindrance often seen in standard 100 Å columns.

150 mm Length – Maximum Resolution for Biomolecules

• Highest resolving power in the 2.1 mm wide‑pore C18 range

• Maximizes peak capacity for complex biomolecule mixtures

• Ideal for proteins, peptides, and closely related macromolecules

• Preferred for LC‑MS workflows requiring detailed separation

• Longer run times compared to 50 mm and 100 mm formats

This column is best suited when maximum separation and purity are required over speed.

Flow Rate & System Requirements (2.1 mm ID, 3 µm, 300 Å)

• Typical flow rate range: ~0.2–0.4 mL/min

• Optimal starting range: ~0.18–0.30 mL/min for maximum resolution

• Requires:

  • HPLC or LC‑MS system capable of stable low‑flow delivery

  • Low‑volume detector flow cell for optimal sensitivity

  • Precision injection system

⚠️ Standard HPLC systems are compatible but must maintain consistent low‑flow control. ⚠️ Monitor pressure when using viscous solvents and long gradients.

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Enables separation of:

  • Proteins

  • Peptides

  • Oligonucleotides

• Eliminates restricted diffusion seen in 100 Å columns

Improved Recovery & Peak Shape

• Better analyte interaction with stationary phase

• Improved peak symmetry and reproducibility for high molecular weight compounds

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic retention behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (3 µm) Performance Advantages

Balanced Efficiency & Pressure

• More efficient than 5 µm columns

• Lower backpressure than 1.8 µm UHPLC columns

• Ideal balance between performance and system compatibility

USP Method Compatibility

• Suitable for USP L1 methods adapted for large molecule separations

• Strong alternative for wide‑pore reversed‑phase methods

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for polar and basic analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced run‑to‑run variation

Cost‑Efficient Operation

• Lower pressure vs UHPLC columns

• Reduced solvent consumption with 2.1 mm format

• Lower cost per analysis

Typical Applications

• Protein and peptide separations

• Oligonucleotide analysis

• Biomolecule LC‑MS workflows

• Lipids and hydrophobic compounds

• Long‑chain fatty acids

• Complex biomolecule mixtures requiring maximum resolution

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Requires stable low‑flow analytical system capability

• Ideal when 100 Å columns limit analyte access or separation

• Optimize temperature and mobile phase conditions for best biomolecule performance