HPLC Column, RP C18, 3um, 2.1mm ID x 150mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 68318‑15P‑2 Cogent RP C18 HPLC Column is designed for maximum‑resolution separations, utilizing 3 µm particles and fully double end‑capped C18 bonding to deliver high efficiency, excellent peak shape, and dependable performance.
Manufactured with high‑purity silica and traditional octadecylsilane (C18) bonding, this stationary phase:
Uses complete end‑capping to minimize silanol interactions
Improves peak symmetry for polar and basic analytes
Provides consistent and reproducible chromatography
This column is widely used as a primary analytical solution and as an alternate column for USP L1 methods using 3 µm particle phases.
150 mm Length – Maximum Resolution for LC‑MS
Highest resolving power within the 2.1 mm RP C18 range
Maximizes peak capacity for complex mixtures
Ideal for separating closely related or co‑eluting compounds
Common choice for LC‑MS workflows requiring enhanced resolution
Longer run times compared to 50 mm and 100 mm formats
This format is best suited when maximum separation performance is required over speed.
Flow Rate & System Requirements (2.1 mm ID, 3 µm)
Typical flow rate range: ~0.2–0.4 mL/min
Optimal starting range: ~0.20–0.30 mL/min for maximum resolution
Requires:
HPLC or LC‑MS system capable of stable low‑flow delivery
Low‑volume detector flow cell
Accurate injection system
⚠️ Standard HPLC systems are compatible but must be capable of consistent low‑flow operation. ⚠️ Monitor pressure when using viscous solvents or extended gradients.
Why Choose RP C18 (3 µm)
Balanced Efficiency & Pressure
Higher efficiency than 5 µm columns
Lower backpressure than 1.8 µm UHPLC columns
Ideal balance between performance and instrument compatibility
Reliable Hydrophobic Retention
Strong reversed‑phase retention for hydrophobic and neutral compounds
Excellent for long‑chain fatty acids and similar analytes
Method Development Versatility
Suitable for new method development
Flexible across a wide range of analytes
Compatible with USP L1 method substitution
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances performance for basic analytes
High Purity Silica
Consistent, reproducible results
Reduced variability between runs and columns
Cost‑Efficient Operation
Lower pressure requirements vs UHPLC columns
Reduced solvent consumption with 2.1 mm ID
Lower cost per analysis
Typical Applications
LC‑MS analytical workflows
Pharmaceutical and QC analysis
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Method development and optimization
USP L1 analytical methods
Important Usage Notes
Designed for low‑flow (2.1 mm) analytical workflows
Requires stable low‑flow system capability
Ideal for complex separations requiring maximum resolution
Preferred when shorter columns do not provide adequate separation