CUSTOM ITEM: HPLC Column, RP C18, Wide Pore, 3um, 4.0mm ID x 100mm Length, 300A. Cogent Brand. 1 EA.
Additional Info:
The 68318‑10P‑4‑3M Cogent RP C18 Wide Pore HPLC Column is engineered for enhanced separation of large molecular weight analytes, combining 3 µm particle size with a 300 Å wide‑pore structure for improved analyte accessibility and performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar compounds
Excellent reproducibility across runs and methods
The 300 Å pore size enables large molecules to fully access the stationary phase, eliminating steric exclusion effects often encountered with standard 100 Å columns.
100 mm Length – Increased Resolution for Biomolecules
Higher resolving power compared to 50 mm columns
Improved peak capacity for large and complex analytes
Better separation of proteins, peptides, and biomolecules
Ideal for method development and LC‑MS workflows
This configuration is preferred when greater resolution is required without the extended run times of longer columns.
4.0 mm ID – Solvent‑Saving Analytical Performance
Comparable performance to 4.6 mm ID columns
Reduced mobile phase consumption and waste
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.9–1.1 mL/min
Benefits:
Lower operating costs
Reduced solvent usage
Compatible with standard HPLC systems
Why Choose Wide Pore (300 Å) C18
Designed for Large Molecules
Suitable for:
Proteins
Peptides
Oligonucleotides
Eliminates limited diffusion issues seen in 100 Å columns
Improved Recovery & Peak Shape
Enhanced interaction with stationary phase
Better peak symmetry for large molecular weight analytes
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic retention
Suitable for lipids and long‑chain fatty acids
RP C18 (3 µm) Performance Advantages
Balanced Efficiency & Pressure
Higher efficiency than 5 µm columns
Lower backpressure than 1.8 µm UHPLC columns
Ideal for standard HPLC systems
USP Method Compatibility
Suitable for USP L1 methods requiring wide‑pore columns
Excellent alternate column for biomolecule applications
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Reduces secondary interactions
Improves peak symmetry
Better performance for polar and basic compounds
High Purity Silica
Consistent chromatography
Reduced variability between runs
Cost‑Efficient Operation
Lower pressure demands vs UHPLC columns
Reduced solvent consumption vs larger ID columns
Lower cost per analysis
Typical Applications
Protein and peptide separations
Oligonucleotide analysis
Biomolecule LC‑MS workflows
Lipids and hydrophobic analytes
Long‑chain fatty acids
Methods requiring wide‑pore reversed‑phase chromatography
Important Usage Notes
Designed for large molecule separations using 300 Å pore media
Compatible with standard HPLC systems
Optimize temperature and mobile phase for best biomolecule performance
Ideal when 100 Å columns limit analyte access or recovery
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable