Catalog Number - 68318-10P-3M

The 68318‑10P‑3M Cogent RP C18 Wide Pore HPLC Column is engineered for enhanced separation of large molecular weight analytes, combining 3 µm particle size with a 300 Å wide‑pore structure for optimal analyte accessibility and chromatographic performance.

Manufactured with high‑purity silica and traditional octadecylsilane (C18) bonding, and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large hydrophobic analytes

• High reproducibility and robustness

The 300 Å pore structure allows large molecules to access the full surface area of the stationary phase, eliminating steric exclusion limitations common with standard 100 Å columns.

100 mm Length – Increased Resolution for Biomolecules

• Higher resolving power compared to 50 mm columns

• Improved separation of large and closely related biomolecules

• Increased peak capacity for complex samples

• Ideal for analytical HPLC and LC‑MS workflows requiring enhanced resolution

This format is often selected when greater separation is needed but 150 mm columns are not required.

4.6 mm ID – Standard Analytical Performance

• Industry‑standard analytical format

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Strong detector signal response

• Robust reproducibility

• Easy method transfer across laboratories

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Enables full interaction for:

  • Proteins

  • Peptides

  • Oligonucleotides

• Eliminates limited mass transfer seen in 100 Å columns

Improved Recovery & Peak Shape

• Better analyte penetration into stationary phase

• Enhanced peak symmetry and reproducibility

Maintains Classic C18 Selectivity

• Retains hydrophobic separation behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (3 µm) Performance Advantages

Balanced Efficiency & Pressure

• Higher efficiency than 5 µm columns

• Lower backpressure than 1.8 µm UHPLC columns

• Ideal for standard HPLC systems

USP Method Compatibility

• Suitable for USP L1 methods adapted for large molecules

• Excellent alternate column for biomolecule separations

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Reduced secondary interactions

• Improved peak symmetry

• Better performance for polar and basic analytes

High Purity Silica

• Consistent chromatography

• Reduced variability between analyses

Cost‑Efficient Operation

• Lower pressure requirements vs UHPLC columns

• Reduced solvent waste compared to longer columns

• Lower cost per analysis

Typical Applications

• Protein and peptide separations

• Oligonucleotide analysis

• Biomolecule HPLC and LC‑MS workflows

• Lipids and hydrophobic compounds

• Long‑chain fatty acids

• Methods requiring wide‑pore reversed‑phase chromatography

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Compatible with standard HPLC systems

• Optimize temperature and mobile phase for best biomolecule performance

• Ideal when standard 100 Å columns cannot effectively separate large analytes