Catalog Number - 68318-10P-2-3M

The 68318‑10P‑2‑3M Cogent RP C18 Wide Pore HPLC Column is engineered for enhanced separation of large molecular weight analytes, combining 3 µm particle size with a 300 Å pore structure to maximize analyte accessibility and chromatographic performance.

This stationary phase is produced using high‑purity silica with traditional octadecylsilane (C18) bonding, and features full double end‑capping, delivering:

• Reduced silanol activity

• Improved peak shape for large, polar, and biomolecular analytes

• Excellent reproducibility and robustness

The 300 Å wide‑pore design allows large molecules to fully access the stationary phase, eliminating steric limitations common with standard 100 Å columns.

100 mm Length – Increased Resolution for Biomolecules

• Higher resolving power than 50 mm columns

• Improved separation of peptides, proteins, and large analytes

• Increased peak capacity for complex mixtures

• Ideal for LC‑MS workflows requiring enhanced resolution

This format is preferred when greater separation is required without the longer run times of 150 mm columns.

Flow Rate & System Requirements (2.1 mm ID, 3 µm, 300 Å)

• Typical flow rate range: ~0.2–0.4 mL/min

• Optimal starting range: ~0.25–0.35 mL/min

• Requires:

  • HPLC or LC‑MS system capable of stable low‑flow delivery

  • Low‑volume detector flow cell

  • Precision injection system

⚠️ Standard HPLC systems are acceptable but must be capable of consistent low‑flow operation for optimal performance.

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Allows access for:

  • Proteins

  • Peptides

  • Oligonucleotides

• Eliminates restricted diffusion seen in 100 Å columns

Improved Recovery & Peak Shape

• Enhanced analyte interaction with stationary phase

• Improved peak symmetry and reproducibility for biomolecules

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (3 µm) Performance Advantages

Balanced Efficiency & Pressure

• Higher efficiency than 5 µm columns

• Lower backpressure than 1.8 µm UHPLC columns

• Ideal for standard HPLC and LC‑MS systems

USP Method Compatibility

• Suitable for USP L1 methods adapted for large molecules

• Reliable alternate column option

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Reduces secondary interactions

• Improves peak symmetry

• Better performance for polar and basic analytes

High Purity Silica

• Consistent chromatography

• Reduced run‑to‑run variability

Cost‑Efficient Operation

• Lower pressure vs UHPLC columns

• Reduced solvent consumption with 2.1 mm ID

• Lower cost per analysis

Typical Applications

• Protein and peptide separations

• Oligonucleotide analysis

• Biomolecule LC‑MS workflows

• Lipids and hydrophobic analytes

• Long‑chain fatty acids

• Methods requiring wide‑pore reversed‑phase chromatography

Important Usage Notes

• Designed for large molecule separations (300 Å pore size)

• Requires stable low‑flow analytical system performance

• Ideal when 100 Å columns limit analyte access or resolution

• Optimize temperature and mobile phase for best biomolecule performance