HPLC Column, RP C18, 3um, 2.1mm ID x 100mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 68318‑10P‑2 Cogent RP C18 HPLC Column delivers premium, balanced performance using 3 µm particles and fully double end‑capped C18 bonding, providing high efficiency, excellent peak shape, and reliable reproducibility.
Manufactured with high‑purity silica and traditional octadecylsilane (C18) bonding, this stationary phase:
Uses complete end‑capping to minimize silanol activity
Improves peak shape for polar and basic analytes
Provides excellent lot‑to‑lot reproducibility
This column is an ideal choice as an alternate for USP L1 methods and as a dependable platform for new method development.
100 mm Length – Increased Resolution for LC‑MS
Higher resolving power compared to 50 mm columns
Improved peak capacity and separation of closely related analytes
Ideal for LC‑MS workflows requiring enhanced resolution
Balanced performance between analysis time and separation quality
This format is commonly selected when greater separation is needed without moving to longer (150 mm) columns.
Flow Rate & System Requirements (2.1 mm ID, 3 µm)
Typical flow rate range: ~0.2–0.4 mL/min
Optimal starting range: ~0.25–0.35 mL/min
Requires:
HPLC system capable of stable low‑flow delivery
Low‑volume detector flow cell for optimal sensitivity
Accurate injection system
⚠️ Standard HPLC systems are generally compatible but must be capable of consistent low‑flow operation.
Why Choose RP C18 (3 µm)
Balanced Efficiency & Pressure
More efficient than 5 µm columns
Lower backpressure than sub‑2 µm UHPLC columns
Ideal balance of performance and system compatibility
Reliable Hydrophobic Retention
Strong retention for hydrophobic and neutral compounds
Excellent for long‑chain fatty acids and similar analytes
Versatile Method Development
Highly adaptable across different methods
Common first‑choice column for new methods
Suitable for USP L1 method substitution
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances performance for basic compounds
High Carbon Load
Increased retention and loading capacity
Improved robustness across diverse analytes
Low Column Bleed
Ideal for sensitive detection methods (LC‑MS)
Cleaner baselines and more accurate data
High Purity Silica
Consistent, reproducible results
Reduced variability between runs and batches
Typical Applications
LC‑MS analytical workflows
Pharmaceutical and QC analysis
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Method development and optimization
USP L1 reversed‑phase methods
Important Usage Notes
Designed for low‑flow (2.1 mm) analytical methods
Requires stable low‑flow system performance
Ideal when increased resolution is needed without UHPLC pressure requirements
Common choice for LC‑MS applications requiring higher separation power