CUSTOM ITEM: HPLC Column, RP C18, Wide Pore, 3um, 4.0mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.
Additional Info:
The 68318‑05P‑4‑3M Cogent RP C18 Wide Pore HPLC Column is a high‑value reversed‑phase column specifically designed for large molecules and biomolecules, combining 3 µm particle size with a 300 Å pore structure for optimal analyte accessibility.
This stationary phase is manufactured with high‑purity silica and traditional octadecylsilane (C18) bonding, with complete double end‑capping, providing:
Reduced silanol interactions
Improved peak shape for proteins and large polar analytes
Consistent and reproducible performance
The wide‑pore (300 Å) design allows larger molecules to fully access the stationary phase, improving retention and recovery compared to standard 100 Å columns.
50 mm Length – Fast Biomolecule Analysis
Optimized for rapid separations and short run times
Lower backpressure than longer columns
Ideal for screening and high‑throughput workflows
Excellent for method development of biomolecules
4.0 mm ID – Solvent‑Saving Analytical Performance
Comparable chromatographic behavior to 4.6 mm ID columns
Reduced mobile phase consumption
Typical flow rate range: ~0.8–1.2 mL/min
Optimal starting range: ~0.9–1.1 mL/min
Benefits:
Lower operating cost
Reduced solvent waste
Compatible with standard HPLC systems
Why Choose Wide Pore (300 Å) C18
Designed for Large Molecules
Provides access to the stationary phase for:
Proteins
Peptides
Oligonucleotides
Prevents steric exclusion seen in 100 Å phases
Improved Recovery & Reproducibility
Better analyte interaction with the stationary phase
Improved peak shapes for large molecular weight compounds
Maintains Reversed‑Phase Selectivity
Retains hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
RP C18 (3 µm) Performance Advantages
Balanced Efficiency & Pressure
More efficient than 5 µm columns
Lower backpressure than sub‑2 µm UHPLC columns
Allows use on standard HPLC systems
USP Method Compatibility
Suitable for USP L1 methods (large molecule adaptation)
Ideal alternate column for wide‑pore requirements
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Improved peak symmetry
Reduced secondary interactions
High Purity Silica
Consistent performance across runs
Reduced variability
Cost‑Efficient Operation
Lower pressure requirements than UHPLC columns
Reduced solvent consumption vs 4.6 mm ID formats
Typical Applications
Protein and peptide separations
Oligonucleotide analysis
Large biomolecule chromatography
Lipids and hydrophobic compounds
Long‑chain fatty acids
Methods requiring wide‑pore reversed‑phase columns
Important Usage Notes
Designed for large molecule separations using wide‑pore media
Compatible with standard HPLC systems
Optimize temperature and mobile phase for best biomolecule performance
Ideal for applications where 100 Å columns limit analyte access
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable