Catalog Number - 68318-05P-3M

The 68318‑05P‑3M Cogent RP C18 Wide Pore HPLC Column is a high‑value reversed‑phase column designed specifically for large molecular weight analytes, combining 3 µm particle size with a 300 Å wide‑pore structure to maximize analyte accessibility and recovery.

Manufactured with high‑purity silica and traditional octadecylsilane (C18) bonding, this stationary phase is fully double end‑capped, providing:

• Reduced silanol activity

• Improved peak shape for large and polar analytes

• Consistent, reproducible chromatography

The 300 Å pore size allows large molecules to fully interact with the stationary phase, eliminating the steric limitations often encountered with standard 100 Å phases.

50 mm Length – Fast Biomolecule Screening

• Designed for rapid separations and short run times

• Lower backpressure compared to longer columns

• Ideal for high‑throughput screening (HTS)

• Excellent for method development of large molecules

4.6 mm ID – Standard Analytical Performance

• Industry‑standard analytical column dimension

• Compatible with all conventional HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Optimal starting range: ~1.0–1.2 mL/min

Provides:

• Robust detector response

• Excellent reproducibility

  Easy method transfer

Why Choose Wide Pore (300 Å) C18

Designed for Large Molecules

• Enables full interaction with:

  • Proteins

  • Peptides

  • Oligonucleotides

• Prevents restricted diffusion seen in 100 Å phases

Improved Recovery & Peak Shape

• Better access to stationary phase

• Improved peak symmetry for biomolecules

• Enhanced reproducibility

Hydrophobic Retention Maintained

• Retains classic reversed‑phase C18 behavior

• Suitable for lipids and long‑chain fatty acids

RP C18 (3 µm) Performance Advantages

Balanced Efficiency & Pressure

• Higher efficiency than 5 µm columns

• Lower backpressure than sub‑2 µm UHPLC columns

• Ideal for standard HPLC systems

USP Method Compatibility

• Suitable for USP L1 methods requiring wide‑pore columns

• Excellent alternate column for large molecule methods

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Reduced secondary interactions

• Improved peak symmetry

• Better performance for polar and basic compounds

High Purity Silica

• Consistent chromatography

• Reduced variability across runs

Cost‑Efficient Operation

• Lower pressure requirements vs UHPLC columns

• Reduced solvent waste with shorter length

• Lower cost per analysis

Typical Applications

• Protein and peptide separations

• Oligonucleotide analysis

• Large biomolecule chromatography

• Lipids and hydrophobic compounds

• Long‑chain fatty acids

• Methods requiring wide‑pore reversed‑phase chromatography

Important Usage Notes

• Designed for large molecule separations using 300 Å pore media

• Compatible with standard HPLC systems

• Optimize temperature and mobile phase to fine‑tune biomolecule separations

• Ideal when 100 Å columns limit analyte access or recovery