HPLC Column, RP C18, 3um, 2.1mm ID x 50mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 68318‑05P‑2 Cogent RP C18 HPLC Column is a high‑value, high‑performance reversed‑phase column featuring 3 µm particles and fully double end‑capped C18 bonding, delivering reliable retention, improved efficiency, and consistent chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this column:
Uses complete end‑capping to minimize silanol interactions
Improves peak shape for polar and basic analytes
Provides consistent and reproducible separations
This column is ideal both as a primary method development tool and as an alternate column for USP L1 methods using 3 µm particles.
50 mm Length – Speed & Throughput
Designed for fast separations and short run times
Lower backpressure compared to longer columns
Ideal for high‑throughput screening and method development
Excellent for LC‑MS workflows requiring rapid analysis
Flow Rate & System Requirements (2.1 mm ID, 3 µm)
Typical flow rate range: ~0.2–0.4 mL/min
Optimal starting range: ~0.25–0.35 mL/min
Requires:
HPLC system capable of stable low‑flow delivery
Low‑volume detector flow cell
Accurate injection system
⚠️ While compatible with standard HPLC systems, longer columns or viscous mobile phases may increase backpressure.
Why Choose RP C18 (3 µm)
Balanced Efficiency & Pressure
Higher efficiency than 5 µm columns
Lower backpressure than sub‑2 µm UHPLC columns
Ideal balance between performance and system compatibility
Reliable Hydrophobic Retention
Strong reversed‑phase retention for hydrophobic and neutral compounds
Excellent for long‑chain fatty acids and similar analytes
USP Method Compatibility
Suitable for USP L1 methods with 3 µm specifications
Excellent alternate column option
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Reduces secondary interactions
Improves peak symmetry
Better performance for basic compounds
High Purity Silica
Consistent, reproducible chromatography
Reduced batch‑to‑batch variation
Cost‑Efficient Operation
Lower pressure vs UHPLC columns
Compatible with a wider range of systems
Lower cost per analysis
Typical Applications
LC‑MS analytical workflows
Pharmaceutical and QC analysis
Hydrophobic and neutral compound separations
Long‑chain fatty acids
Method development and validation
USP L1 method applications
Important Usage Notes
Designed for low‑flow (2.1 mm) analytical methods
Requires stable low‑flow system performance
Monitor pressure for viscous solvents or longer column methods
Ideal balance between efficiency and instrument compatibility