Catalog Number - 68308-15P-2

Cogent RP C8 Octyl™ is a versatile reversed-phase HPLC stationary phase designed for the separation of hydrophobic and neutral compounds. Manufactured using a traditional octyl (C8) bonded phase on ultra-high purity silica and fully double end capped, it provides strong hydrophobic interactions while often offering reduced retention compared to C18 phases. As a USP L7 equivalent phase, it is widely used for method replacement, method development, and routine analytical separations where balanced retention and selectivity are desired.

68308-15P-2 is an HPLC column packed with 3 µm RP C8 Octyl™ particles featuring a 100 Å pore size in a 2.1 mm ID x 150 mm format. This narrow-bore configuration combines maximum chromatographic resolution with reduced solvent consumption and excellent compatibility with LC-MS instrumentation. The 150 mm length provides significantly greater peak capacity and resolving power than shorter column formats, while the 3 µm particles deliver higher efficiency and sharper peaks than comparable 5 µm packings. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, standard HPLC systems must be capable of precise and reproducible low-flow operation.

This column is particularly useful for hydrophobic and neutral compounds in reversed-phase chromatography and is especially well suited for long-chain fatty acid analysis. The C8 chemistry offers excellent retention and selectivity while often reducing excessive retention compared to more hydrophobic phases. This column format is frequently selected for LC-MS methods when maximum resolution and peak capacity are required.

Key Benefits

• RP C8 Octyl™ stationary phase

• USP L7 equivalent chemistry

• Fully double end capped

• Traditional octyl (C8) bonded phase

• Ultra-high purity silica support

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Maximum peak capacity

• Excellent for LC-MS applications

• Reduced solvent consumption

Typical Applications

• USP L7 methods

• Reversed-phase HPLC

• LC-MS methods

• Long-chain fatty acid analysis

• Hydrophobic compound analysis

• Neutral compound analysis

• Pharmaceutical analysis

• Method development

• Impurity profiling

• Complex sample separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8 Octyl™

• USP Classification: L7

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Length: 150 mm

• Typical Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Suitable for conventional HPLC and LC-MS systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 2.1 mm ID format is commonly used for LC-MS applications

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.3 mL/min

• Detector flow cells should be optimized for low-volume, narrow-bore chromatography

• The 150 mm length maximizes resolution and peak capacity for complex separations

• Particularly useful for long-chain fatty acid separations

• Methods using viscous mobile phases may approach instrument pressure limits

• Commonly used as an alternative for USP L7 methods