Catalog Number - 68308-10P

Cogent RP C8 Octyl™ is a versatile reversed-phase HPLC stationary phase designed for the separation of hydrophobic and neutral compounds. Manufactured using a traditional octyl (C8) bonded phase on ultra-high purity silica and fully double end capped, it provides strong hydrophobic interactions while often offering reduced retention compared to C18 phases. As a USP L7 equivalent phase, it is widely used for method replacement, method development, and routine analytical separations where balanced retention and selectivity are desired.

68308-10P is an HPLC column packed with 3 µm RP C8 Octyl™ particles featuring a 100 Å pore size in a 4.6 mm ID x 100 mm format. This column length is often selected when greater resolution is required than a 50 mm column can provide while still maintaining relatively short analysis times. The 3 µm particles deliver higher efficiency and sharper peaks than comparable 5 µm columns, providing an excellent balance of speed and chromatographic performance. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.

This column is particularly useful for hydrophobic and neutral compounds in reversed-phase chromatography and is especially well suited for long-chain fatty acid analysis. The C8 chemistry offers excellent retention and selectivity while often reducing excessive retention compared to more hydrophobic phases, making it an excellent choice for both routine and method development applications.

Key Benefits

• RP C8 Octyl™ stationary phase

• USP L7 equivalent chemistry

• Fully double end capped

• Traditional octyl (C8) bonded phase

• Ultra-high purity silica support

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Improved chromatographic resolution

• Balanced retention characteristics

• Excellent for long-chain fatty acid analysis

Typical Applications

• USP L7 methods

• Reversed-phase HPLC

• Long-chain fatty acid analysis

• Hydrophobic compound analysis

• Neutral compound analysis

• Pharmaceutical analysis

• Method development

• Quality control testing

• Routine analytical methods

• Preparative method scouting

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8 Octyl™

• USP Classification: L7

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 100 mm

• Length: 100 mm

• Typical Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• Suitable for conventional HPLC systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 100 mm length provides greater resolution than 50 mm columns while maintaining relatively short run times

• Particularly useful for long-chain fatty acid separations

• Commonly used for hydrophobic and neutral compounds

• Methods using longer columns and viscous mobile phases may approach instrument pressure limits

• Commonly used as an alternative for USP L7 methods