HPLC Column, RP C8 Octyl, Wide Pore, 3um, 2.1mm ID x 50mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C8 Octyl™ Wide Pore is a reversed-phase HPLC stationary phase specifically designed for the separation of large hydrophobic and neutral molecules. Manufactured using a traditional octyl (C8) bonded phase on ultra-high purity silica and fully double end capped, this 300 Å wide pore chemistry provides enhanced accessibility for larger analytes compared to standard 100 Å pore columns. As a USP L7 equivalent phase for large molecule applications, it is well suited for biomolecules, long-chain fatty acids, and other compounds that require larger pore structures for optimal chromatographic performance.
68308-05P-2-3M is an HPLC column packed with 3 µm RP C8 Octyl™ Wide Pore particles featuring a 300 Å pore size in a 2.1 mm ID x 50 mm format. This narrow-bore configuration is designed for rapid separations while minimizing solvent consumption and is particularly attractive for LC-MS applications. The 300 Å pore structure allows larger molecules to efficiently access the bonded phase, while the 3 µm particles provide excellent efficiency and peak shape. Typical operating flow rates are approximately 0.2–0.4 mL/min. Because of the low flow rates required by 2.1 mm ID columns, HPLC systems must be capable of precise and reproducible low-flow operation.
This column is particularly useful for large hydrophobic or neutral compounds analyzed by reversed-phase HPLC and LC-MS. The wide pore design provides improved mass transfer for larger analytes, and the C8 chemistry offers balanced hydrophobic retention suitable for biomolecules, long-chain fatty acids, and other large molecular weight compounds.
Key Benefits
RP C8 Octyl™ Wide Pore stationary phase
USP L7 equivalent chemistry
Fully double end capped
Traditional octyl (C8) bonded phase
Ultra-high purity silica support
300 Å wide pore design
Optimized for large molecules
3 µm particle technology
Reduced solvent consumption
Excellent for LC-MS applications
Typical Applications
USP L7 methods
LC-MS methods
Protein analysis
Peptide analysis
Long-chain fatty acid analysis
Large molecule separations
Biopharmaceutical analysis
Hydrophobic compound analysis
Method development
Reversed-phase HPLC
Specifications
Product Type: HPLC Column
Stationary Phase: RP C8 Octyl™ Wide Pore
USP Classification: L7
Chromatography Mode: Reversed Phase
Bonded Phase: Octyl (C8)
Endcapping: Fully Double End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 2.1 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 0.2–0.4 mL/min
Brand: Cogent
Important Notes
Designed for large hydrophobic and neutral molecules
300 Å wide pores improve access to the bonded phase for larger analytes
Suitable for conventional HPLC and LC-MS systems
The 2.1 mm ID format is commonly used for LC-MS applications
Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.4 mL/min
Detector flow cells should be optimized for low-volume, narrow-bore chromatography
The short 50 mm length provides rapid analysis and increased throughput
Particularly useful for long-chain fatty acids and large molecular weight compounds
Commonly used as an alternative for USP L7 methods involving larger analytes