Catalog Number - 68308-05P

Cogent RP C8 Octyl™ is a versatile reversed-phase HPLC stationary phase designed for the separation of hydrophobic and neutral compounds. Manufactured using a traditional octyl (C8) bonded phase on ultra-high purity silica and fully double end capped, it provides strong hydrophobic interactions while offering lower retention than C18 phases in many applications. As a USP L7 equivalent phase, it is widely used for method replacement, method development, and routine analytical separations where balanced retention and selectivity are desired.

68308-05P is an HPLC column packed with 3 µm RP C8 Octyl™ particles featuring a 100 Å pore size in a 4.6 mm ID x 50 mm format. The short 50 mm length is optimized for rapid analyses and increased sample throughput, while the 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and instrument configuration.

This column is particularly useful for hydrophobic and neutral compounds in reversed-phase chromatography. The C8 chemistry is especially well suited for long-chain fatty acids and compounds that may exhibit excessive retention on more hydrophobic phases. It is also frequently chosen as a scouting column during preparative method development because of its balanced retention characteristics and efficient separations.

Key Benefits

• RP C8 Octyl™ stationary phase

• USP L7 equivalent chemistry

• Fully double end capped

• Traditional octyl (C8) bonded phase

• Ultra-high purity silica support

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Sharp peak shapes

• Fast analysis times

• Excellent scouting column for preparative methods

Typical Applications

• USP L7 methods

• Reversed-phase HPLC

• Long-chain fatty acid analysis

• Hydrophobic compound analysis

• Neutral compound analysis

• Pharmaceutical analysis

• Method development

• Preparative method scouting

• Quality control testing

• Routine analytical methods

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8 Octyl™

• USP Classification: L7

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 50 mm

• Length: 50 mm

• Typical Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• Suitable for conventional HPLC systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 50 mm length is ideal for rapid analyses and high-throughput workflows

• Commonly used for hydrophobic and neutral compounds

• Particularly useful for long-chain fatty acid separations

• Frequently selected as a scouting column for preparative method development

• Commonly used as an alternative for USP L7 methods

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