Catalog Number - 68124-15P-2

Cogent RP Phenyl™ is a reversed-phase UHPLC stationary phase designed to provide enhanced selectivity for aromatic and structurally related compounds. Manufactured using a propyl linker with a terminal phenyl ring bonded to ultra-high purity silica and fully double end capped, this phase offers unique π-π interaction mechanisms that can improve the separation of compounds that may be difficult to resolve on traditional alkyl phases. It is particularly useful for aromatic pharmaceuticals, positional isomers, flavonoids, tocopherols, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, and many natural products.

68124-15P-2 is a UHPLC column packed with 1.8 µm RP Phenyl™ particles featuring a 100 Å pore size in a 2.1 mm ID x 150 mm format. The 150 mm length maximizes resolution and peak capacity for complex sample separations, while the sub-2 µm particle technology delivers exceptional chromatographic efficiency. This column is specifically engineered for high-pressure UHPLC applications and is designed to take full advantage of UHPLC instrumentation capable of operating at the elevated pressures generated by 1.8 µm particles. Typical operating flow rates are approximately 0.2–0.4 mL/min. Because of the low flow rates required by 2.1 mm ID columns, any HPLC system used with this column must be capable of accurate and reproducible low-flow operation.

This column is frequently selected for LC-MS methods involving complex sample matrices where maximum resolving power is required. The combination of RP Phenyl™ selectivity and UHPLC efficiency makes it an excellent choice for aromatic compounds, positional isomers, APIs, and natural products requiring enhanced chromatographic resolution.

Key Benefits

• RP Phenyl™ stationary phase

• Fully double end capped

• Propyl phenyl bonded phase

• Ultra-high purity silica support

• Enhanced aromatic selectivity

• π-π interaction capability

• Designed for UHPLC applications

• High-efficiency 1.8 µm particles

• Maximum peak capacity

• Ideal for LC-MS methods

Typical Applications

• LC-MS methods

• Positional isomer separations

• Tocopherol analysis

• Flavonoid analysis

• Polynuclear aromatic hydrocarbon analysis

• Nitro-aromatic compound analysis

• API analysis

• Natural product analysis

• Pharmaceutical method development

• Complex sample separations

Specifications

• Product Type: UHPLC Column

• Stationary Phase: RP Phenyl™

• Chromatography Mode: Reversed Phase

• Bonded Phase: Propyl Phenyl

• Endcapping: Fully Double End Capped

• Particle Size: 1.8 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 150 mm

• Length: 150 mm

• Typical Flow Rate: 0.2–0.4 mL/min

• Brand: Cogent

Important Notes

• Specifically designed for UHPLC operation and high-pressure reversed-phase chromatography

• Delivers maximum performance when used on a UHPLC system designed for sub-2 µm particle columns

• While compatible HPLC systems may be used in some applications, performance advantages are realized on UHPLC systems capable of operating at the required pressures

• Sub-2 µm particles generate significantly higher backpressure than conventional HPLC columns

• The 2.1 mm ID format is commonly used for LC-MS applications and reduced solvent consumption

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.4 mL/min

• The 150 mm length maximizes resolution and peak capacity for complex separations

• Enhanced selectivity for aromatic and phenyl-containing compounds compared to traditional alkyl phases

• Samples should be filtered through a 0.2 µm syringe filter prior to injection

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