UHPLC Column, RP C18, 1.8um, 100A. 2.1mm ID x 100mm Length. Cogent Brand. 1 EA.

Additional Info:

The 68118‑10P‑2 Cogent RP C18 UHPLC Column is a high‑efficiency reversed‑phase column featuring 1.8 µm particles and fully double end‑capped C18 bonding, delivering maximum resolution, peak capacity, and method reliability.

Manufactured with high‑purity silica and traditional octadecylsilane (C18) bonding, this column:

  • Minimizes silanol activity through complete end‑capping

  • Improves peak shape for polar and basic analytes

  • Provides highly reproducible and robust chromatographic performance

This column is widely used as a primary method development and LC‑MS column due to its versatility and performance.

100 mm Length – Increased Resolution for LC‑MS

  • Higher resolving power compared to 50 mm columns

  • Improved peak capacity and separation of closely related analytes

  • Ideal for LC‑MS workflows requiring enhanced resolution

  • Balanced performance between speed and separation quality

This is one of the most popular UHPLC column formats, especially for analytical workflows requiring greater resolution without excessive run time.

Flow Rate & System Requirements (2.1 mm ID, 1.8 µm)

⚠️ Requires UHPLC system capability due to high backpressure

  • Typical flow rate range: ~0.2–0.5 mL/min

  • Optimal starting range: ~0.2–0.35 mL/min for resolution

  • Requires:

    • UHPLC system capable of high‑pressure operation

    • Low‑volume detector flow cell

    • Accurate low‑flow pumping

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter before injection to prevent clogging.

⚠️ Standard HPLC systems must be capable of stable low‑flow operation and may not support required pressures.

Why Choose RP C18 for UHPLC

High Efficiency & Resolution

  • 1.8 µm particles provide maximum plate count

  • Greater peak capacity than 3 µm columns

  • Maintains resolution even with shorter run times

Reliable Hydrophobic Retention

  • Ideal for hydrophobic and neutral compounds

  • Strong and reproducible reversed‑phase behavior

Versatile Method Development

  • Widely used across analytical laboratories

  • Excellent first‑choice column for new methods

  • Easily scalable to longer column lengths or different IDs

Cogent RP C18™ Advantages

Fully End‑Capped Surface

  • Minimizes secondary interactions

  • Improves peak symmetry

  • Better performance for basic compounds

High Purity Silica

  • Consistent performance across batches

  • Improved reproducibility

Cost‑Efficient UHPLC Operation

  • Reduced solvent consumption with 2.1 mm ID

  • Increased throughput

  • Lower cost per analysis

Typical Applications

  • LC‑MS analytical workflows

  • Pharmaceutical and QA/QC analysis

  • Hydrophobic and neutral compound separations

  • Method development and optimization

  • High‑resolution UHPLC applications

Important Usage Notes

  • Requires UHPLC system capable of high pressure

  • Use 0.2 µm filtration to protect column integrity

  • Ideal for high‑efficiency, high‑resolution workflows

  • One of the most common formats for LC‑MS applications

 

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