Catalog Number - 68118-05P

The 68118‑05P Cogent RP C18 UHPLC Column is a high‑efficiency reversed‑phase column featuring 1.8 µm particles and fully double end‑capped C18 bonding, providing maximum efficiency, strong retention, and highly reproducible separations.

Manufactured with high‑purity silica and traditional octadecylsilane (C18) bonding, this column is fully end‑capped to:

• Minimize residual silanol activity

• Improve peak shape—especially for polar and basic compounds

• Deliver consistent and reliable chromatographic performance

This makes RP C18 the preferred “first‑choice” column for routine methods, method development, and high‑throughput environments.

50 mm Length – Speed & Throughput

• Designed for fast separations and short run times

• Lower analysis time compared to longer columns

• Ideal for high‑throughput screening (HTS)

• Efficient for rapid method development and optimization

Flow Rate & System Requirements (4.6 mm ID, 1.8 µm)

⚠️ Requires UHPLC system capability due to high backpressure

• Typical flow rate range: ~1.0–2.0 mL/min

• Optimal starting range: ~1.2–1.5 mL/min

• Requires:

  • UHPLC system capable of high‑pressure operation

  • Standard flow detector compatible with 4.6 mm ID

  • Stable high‑flow pumping capability

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection to avoid clogging.

Why Choose RP C18 for UHPLC

Maximum Efficiency at High Speed

• 1.8 µm particles provide high plate counts and peak capacity

• Maintains resolution while significantly reducing run times

• Improves performance compared to 3 µm+ particle columns

Reliable Hydrophobic Retention

• Strong, predictable retention for hydrophobic and neutral compounds

• Ideal for routine analytical applications

Versatile Method Development Platform

• Widely applicable across industries and analyte classes

• Serves as a universal starting point for new methods

• Easy scaling between column lengths and IDs

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Provides better performance for basic analytes

High Purity Silica

• Consistent column‑to‑column performance

• Reduced variability and improved reproducibility

Cost‑Efficient Operation

• Shorter run times = higher throughput

• Reduced solvent waste vs longer columns

• Lower overall operating cost per analysis

Typical Applications

• High‑throughput analytical HPLC

• Pharmaceutical analysis and QA/QC

• Hydrophobic and neutral compound separations

• Method development and optimization

• Routine reversed‑phase workflows

Important Usage Notes

• Requires UHPLC system (high pressure capable)

• Use 0.2 µm filtration to protect column lifetime

• Ideal for fast, high‑efficiency analytical workflows

• Best suited for standard‑flow HPLC systems upgraded to UHPLC capability