Catalog Number - 68108-05P-2

Cogent RP C8 Octyl is a highly versatile reversed-phase HPLC and UHPLC stationary phase designed for the separation of hydrophobic and neutral compounds. Manufactured using a traditional octyl (C8) bonded phase on ultra-high purity silica and fully double end capped, it provides excellent reproducibility, strong hydrophobic retention, and outstanding peak shape. Compared to C4 phases, C8 offers increased retention while often providing faster method development and shorter analysis times than more retentive C18 phases.

68108-05P-2 is a UHPLC column packed with 1.8 µm RP C8 Octyl particles featuring a 100 Å pore size in a 2.1 mm ID x 50 mm format. The short 50 mm length is optimized for rapid separations, while the 1.8 µm particle technology delivers exceptional efficiency, increased peak capacity, and superior chromatographic performance. This column is specifically engineered for high-pressure UHPLC applications and is designed to take full advantage of UHPLC instrumentation capable of operating at the elevated pressures generated by sub-2 µm particles. Typical operating flow rates are approximately 0.2–0.4 mL/min. Because of the low flow rates required by 2.1 mm ID columns, any HPLC system used with this column must be capable of delivering accurate and reproducible low-flow performance.

This column is ideal for hydrophobic and neutral compounds when reducing analysis time while maintaining resolution, or when improving the resolution of existing methods. The 2.1 mm ID format is frequently selected for LC-MS applications due to lower solvent consumption and enhanced mass spectrometer compatibility. While compatible HPLC systems may be used in some applications, this column is designed primarily for UHPLC operation where the full benefits of sub-2 µm particle technology—including higher efficiency, increased peak capacity, improved resolution, and faster separations—can be achieved.

Key Benefits

• RP C8 Octyl stationary phase

• Fully double end capped

• Traditional octyl (C8) bonded phase

• Ultra-high purity silica support

• Engineered for UHPLC applications

• 1.8 µm particle technology

• Exceptional column efficiency

• Increased peak capacity

• Fast analysis times

• Reduced solvent consumption

Typical Applications

• UHPLC analysis

• High-pressure liquid chromatography

• LC-MS methods

• High-throughput laboratories

• Method development

• Hydrophobic compound analysis

• Neutral compound analysis

• Pharmaceutical analysis

• Environmental testing

• Impurity profiling

Specifications

• Product Type: UHPLC Column

• Stationary Phase: RP C8 Octyl

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 1.8 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Typical Flow Rate: 0.2–0.4 mL/min

• Brand: Cogent

Important Notes

• Specifically designed for UHPLC operation and high-pressure reversed-phase separations

• Delivers maximum performance when used on a UHPLC system designed for sub-2 µm particle columns

• May be used on compatible HPLC systems that can reliably operate at the required pressures and low flow rates

• Sub-2 µm particles generate significantly higher backpressure than conventional HPLC columns

• The 2.1 mm ID format is commonly used for LC-MS applications and reduced solvent consumption

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.4 mL/min

• Ideal for reducing run times while maintaining chromatographic resolution

• Samples should be filtered through a 0.2 µm syringe filter prior to injection

• Provides greater retention than C4 phases while often offering faster method development than C18 columns

• Short column length increases sample throughput and laboratory productivity

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