Catalog Number - 68018-SP-PG1-3

The 68018‑SP‑PG1‑3 Cogent RP C18 Wide Pore Guard Column Cartridges are designed to protect semi‑preparative HPLC columns used for large molecule separations, ensuring longer column life and consistent chromatographic performance.

Packed with 10 µm Cogent RP C18™ Wide Pore stationary phase (300 Å), and manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, these cartridges are fully double end‑capped to provide:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible column protection

Constructed in stainless steel tubes with stainless steel frits, these guard cartridges are built for durability in high‑load semi‑prep environments.

Purpose of Guard Columns

• Protect semi‑prep columns from:

  • Particulate contamination

  • Strongly retained biomolecules

  • Matrix interferences and fouling

• Extend column lifetime

• Maintain consistent retention and peak shape

• Reduce downtime and column replacement cost

300 Å Wide Pore – Biomolecule Protection

• Designed specifically for large molecular weight analytes

• Ideal for:

  • Proteins

  • Peptides

  • Biomacromolecules

• Prevents:

  • Pore blockage

  • Restricted diffusion effects seen in 100 Å materials

10 µm Guard Media – Preparative Compatibility

• Matches 10 µm semi‑prep/prep column packing

• Supports high‑loading purification workflows

• Maintains consistent selectivity during scale‑up

10 mm ID – Semi‑Preparative Systems

• Designed for 10 mm ID semi‑prep HPLC columns

• Supports higher flow rates and injection volumes

• Ideal for purification and scale‑up workflows

⚠️ Requires use with a 10 mm ID guard column holder system

Why Choose Wide Pore Guard Cartridges (10 µm, 300 Å)

Matched Chemistry for Biomolecules

• Same wide‑pore C18 chemistry as the column being protected

• Maintains selectivity and retention behavior

• Prevents unintended method changes

Enhanced Protection for Large Molecules

• Traps strongly retained biomolecules before column entry

• Reduces column fouling and surface contamination

Preparative Workflow Alignment

• Ideal for semi‑prep and purification systems

• Supports analytical‑to‑preparative scale‑up consistency

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances stability for biomolecule workflows

High Purity Silica

• Consistent cartridge‑to‑cartridge performance

• Reliable protection across runs

Durable Construction

• Stainless steel housing and frits

• Designed for repeated use in demanding applications

Typical Applications

• Semi‑preparative biomolecule HPLC

• Protein and peptide separations

• Column protection in purification workflows

• Method development and scale‑up

• Complex biological matrices

• USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for 300 Å wide‑pore (biomolecule) applications

• Must be used with a 10 mm ID compatible guard column holder

• Replace regularly to maintain optimal column performance

• Critical for protecting high‑value semi‑prep columns

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.