Catalog Number - 68018-SP50-3M

The 68018‑SP50‑3M Cogent RP C18 Wide Pore Semi‑Prep HPLC Column is designed for biomolecule purification and preparative method development, combining 10 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, high loading capacity, and consistent chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible purification performance

The 300 Å pore size enables large molecules to fully penetrate the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.

This column is ideal as a USP L1 alternate for large molecule applications and as a starting platform for semi‑prep purification workflows and scale‑up.

50 mm Length – Fast Biomolecule Scouting & Purification

• Designed for rapid separations and short run times

• Ideal for early‑stage biomolecule method development

• Reduces solvent consumption during scouting

• Enables high‑throughput purification workflows

10 mm ID – Semi‑Preparative Loading Capacity

• Provides significantly increased loading capacity vs analytical columns

• Ideal for purification and isolation of biomolecules

• Supports semi‑preparative chromatography workflows

Typical Flow Rate Range

• ~2.0–5.0 mL/min (system dependent)

• Optimize based on instrument capability and purification goals

✅ Designed for semi‑preparative HPLC systems capable of higher flow rates and injection volumes

Flow Rate & System Considerations (10 mm ID, 10 µm, 300 Å)

• Requires systems capable of:

  • Higher flow rates

  • Large injection volumes

• Compatible with:

  • Semi‑prep pumps

  • High‑volume injection systems

  • Fraction collection systems

⚠️ Not intended for standard analytical HPLC systems

Why Choose Wide Pore (300 Å) C18 (10 µm, Semi‑Prep)

Designed for Biomolecules

• Enables purification of:

  • Proteins

  • Peptides

  • Large hydrophobic compounds

• Eliminates mass transfer limitations of smaller pore materials

Ideal for Preparative Scouting

• Used to develop and optimize methods prior to preparative scale‑up

• Predictable selectivity when transferring to larger preparative columns

• Supports efficient analytical‑to‑preparative workflows

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for biomolecule separations

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between runs

Durable & Cost‑Efficient

• Long column lifetime

• Ideal for repeated purification cycles

• Lower maintenance requirements

Typical Applications

• Semi‑preparative biomolecule purification

• Protein and peptide separations

• Preparative method scouting

• Hydrophobic and neutral compound separations

• Analytical to preparative scale‑up workflows

• USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for 300 Å wide‑pore biomolecule applications

• Requires semi‑prep HPLC system capability (10 mm ID)

• Ideal for fast screening prior to purification scale‑up

• Optimize for resolution vs throughput balance

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.