Catalog Number - 68018-SP50

The 68018‑SP50 Cogent RP C18 Semi‑Prep HPLC Column is designed for method development and preparative scouting, utilizing 10 µm particles and fully double end‑capped C18 bonding to deliver reliable retention, high loading capacity, and durable chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this stationary phase provides:

• Complete end‑capping to minimize silanol interactions

• Improved peak symmetry for polar and basic analytes

• Consistent and reproducible purification performance

This column is ideal as a USP L1 alternate and as a starting platform for analytical‑to‑preparative method development.

50 mm Length – Fast Scouting & Purification

• Designed for rapid separations and short run times

• Ideal for early‑stage method development and screening

• Minimizes solvent usage during scouting

• Supports high‑throughput purification workflows

10 mm ID – Semi‑Preparative Loading Capacity

• Provides significantly increased loading capacity vs analytical columns

• Ideal for compound isolation and purification

• Supports semi‑preparative chromatography workflows

Typical Flow Rate Range

• ~2.0–5.0 mL/min (system dependent)

• Optimize based on instrument capability and purification goals

✅ Designed for semi‑preparative HPLC systems capable of higher flow rates and injection volumes

Flow Rate & System Considerations (10 mm ID, 10 µm)

• Requires systems capable of higher flow rates and larger injection volumes

• Compatible with:

  • Semi‑prep pumps

  • High‑volume injection systems

  • Fraction collection systems

⚠️ Not intended for standard low‑flow analytical HPLC systems

Why Choose RP C18 (10 µm, Semi‑Prep)

Ideal for Preparative Scouting

• Used to develop and optimize methods prior to preparative scale‑up

• Predictable selectivity when transferring to larger ID preparative columns

• Supports efficient analytical‑to‑preparative workflows

Reliable Hydrophobic Retention

• Strong reversed‑phase retention for hydrophobic and neutral compounds

• Excellent for long‑chain fatty acids

• Provides greater retention than C8 phases

Method Development & USP Compatibility

• Suitable for USP L1 methods

• Ideal for new purification and analytical method development

• Consistent and dependable stationary phase chemistry

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances performance for challenging analytes

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between runs

Durable & Cost‑Efficient

• Long column lifetime

• Ideal for repeated purification cycles

• Reduced maintenance frequency

Typical Applications

• Semi‑preparative purification

• Preparative method scouting

• Hydrophobic and neutral compound separations

• Long‑chain fatty acids

• Analytical to preparative scale‑up workflows

• USP L1 reversed‑phase methods

Important Usage Notes

• Designed for semi‑preparative HPLC systems (10 mm ID)

• Requires systems capable of higher flow rates and loading volumes

• Ideal for fast screening prior to scale‑up

• Choose when loading capacity and robustness are prioritized over efficiency

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.