Semi Prep HPLC Column, RP C18 Wide Pore, 10um, 300A. 10mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68018‑SP100‑3M Cogent RP C18 Wide Pore Semi‑Prep HPLC Column is designed for biomolecule purification and preparative method development, combining 10 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, high loading capacity, and consistent chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Consistent and reproducible purification performance
The 300 Å pore structure allows large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å materials.
This column is ideal as a USP L1 alternate for large molecule applications and as a refinement platform for semi‑prep purification workflows.
100 mm Length – Increased Resolution for Biomolecule Development
Higher resolving power than 50 mm scouting columns
Improved separation of proteins, peptides, and complex biomolecules
Increased peak capacity during method optimization
Ideal for refining purification prior to scale‑up
This format is selected when greater resolution is needed beyond initial scouting runs.
10 mm ID – Semi‑Preparative Loading Capacity
Provides significantly increased loading capacity vs analytical columns
Ideal for biomolecule purification and compound isolation
Supports semi‑preparative chromatography workflows
Typical Flow Rate Range
~2.0–5.0 mL/min (system dependent)
Optimize based on instrument capability and purification goals
✅ Designed for semi‑preparative HPLC systems capable of higher flow rates and injection volumes
Flow Rate & System Considerations (10 mm ID, 10 µm, 300 Å)
Requires systems capable of:
Higher flow rates
Large injection volumes
Compatible with:
Semi‑prep pumps
High‑volume injection systems
Fraction collection systems
⚠️ Not intended for standard analytical HPLC systems
Why Choose Wide Pore (300 Å) C18 (10 µm, Semi‑Prep)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore materials
Method Development to Scale‑Up
Ideal for developing and refining biomolecule purification methods
Predictable selectivity when transitioning to larger preparative columns
Supports efficient analytical‑to‑semi‑prep workflows
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances performance for biomolecule separations
High Purity Silica
Consistent chromatographic performance
Reduced variability between runs
Durable & Cost‑Efficient
Long column lifetime
Ideal for repeated purification cycles
Lower maintenance requirements
Typical Applications
Semi‑preparative biomolecule purification
Protein and peptide separations
Method development and optimization
Hydrophobic and neutral compound isolation
Analytical to preparative scale‑up workflows
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires semi‑prep HPLC system capability (10 mm ID)
Ideal for method refinement prior to scale‑up
Optimize for resolution vs throughput balance
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable