Process HPLC Column, RP C18, Wide Pore, 10um, 300A. 50mm ID x 50mm Length. Cogent Brand. 1 EA.

Additional Info:

The 68018‑P50‑50‑3M Cogent RP C18 Wide Pore Process HPLC Column is designed for industrial‑scale biomolecule purification and process chromatography development, combining 10 µm particles with a 300 Å wide‑pore structure to deliver maximum durability, extremely high loading capacity, and efficient analyte accessibility.

Manufactured using high‑purity 300 Å silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:

  • Reduced silanol interactions

  • Improved peak shape for proteins, peptides, and large polar analytes

  • Consistent and reproducible performance in high‑throughput process workflows

The 300 Å pore structure enables large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to 100 Å media.

This column is ideal as a USP L1 alternate for large‑molecule methods and as a fast‑throughput platform for process‑scale biomolecule purification workflows.

50 mm Length – Fast Biomolecule Scouting & Process Screening

  • Designed for rapid separations and short cycle times

  • Ideal for early‑stage process method development

  • Minimizes solvent consumption in large‑scale screening

  • Enables high‑throughput purification and process optimization

50 mm ID – Maximum Industrial‑Scale Loading

  • Provides extremely high sample loading capacity (top tier across entire column system)

  • Designed for industrial purification, manufacturing workflows, and continuous processing

  • Supports ultra‑high‑flow chromatography systems

Typical Flow Rate Range

  • ~50–150 mL/min (system dependent)

  • Optimize based on process requirements and system capability

✅ Designed for process HPLC systems capable of ultra‑high flow rates and very large injection volumes

Flow Rate & System Considerations (50 mm ID, 10 µm, 300 Å)

  • Requires systems capable of:

    • Ultra‑high flow rates

    • Very large injection volumes

  • Compatible with:

    • Process chromatography systems

    • Industrial pumps and injection platforms

    • Large‑volume fraction collection or continuous processing systems

⚠️ Not suitable for analytical, semi‑prep, or standard preparative HPLC systems

Why Choose Wide Pore (300 Å) C18 (10 µm, Process Scale)

Designed for Biomolecules

  • Enables purification of:

    • Proteins

    • Peptides

    • Large hydrophobic compounds

  • Eliminates mass transfer limitations of smaller pore structures

High‑Throughput Process Purification

  • Ideal for industrial‑scale workflows requiring speed and robustness

  • Maximizes throughput and sample loading capacity

  • Supports efficient purification in manufacturing environments

Scalable Workflow Integration

  • Predictable selectivity across analytical → semi‑prep → prep → production → process

  • Supports seamless method transfer and industrial scale‑up

Cogent RP C18™ Advantages

Fully End‑Capped Surface

  • Minimizes secondary interactions

  • Improves peak symmetry

  • Enhances stability for biomolecule separations at scale

High Purity Silica

  • Consistent chromatographic performance

  • Reduced variability across large‑scale and continuous runs

Durable & Cost‑Efficient

  • Long column lifetime

  • Designed for high‑load industrial workflows

  • Reduces maintenance and operational costs

Typical Applications

  • Industrial‑scale biomolecule purification

  • Protein and peptide separations

  • Process method development and screening

  • Pharmaceutical manufacturing

  • Continuous processing systems

  • USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

  • Designed for 300 Å wide‑pore biomolecule applications

  • Requires process‑scale HPLC system capability (50 mm ID)

  • Ideal for fast screening prior to longer column or full production workflows

  • Optimize for throughput vs resolution depending on process goals

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

  • Orders cannot be canceled once placed

  • Custom items are non‑returnable

© Copyright 2026. MICROSOLV. All Rights Reserved. Website & Hosting by BlueTone Media