Process HPLC Column, RP C18, Wide Pore, 10um, 300A. 50mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68018‑P50‑1003M Cogent RP C18 Wide Pore Process HPLC Column is engineered for industrial‑scale biomolecule purification and process chromatography development, combining 10 µm particles with a 300 Å wide‑pore structure to deliver exceptional durability, extremely high loading capacity, and efficient analyte accessibility under high‑throughput conditions.
Manufactured using high‑purity 300 Å silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Consistent and reproducible performance in large‑scale and continuous purification workflows
The 300 Å pore structure allows large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å materials.
This column is ideal as a USP L1 alternate and as a refinement platform for process‑scale biomolecule purification and manufacturing workflows.
100 mm Length – Increased Resolution for Process Development
Higher resolving power than 50 mm process scouting columns
Improved separation of proteins, peptides, and complex biomolecule mixtures
Increased peak capacity for industrial purification workflows
Ideal for refining methods prior to longer columns or full production runs
This configuration is selected when greater resolution is required beyond initial process‑scale screening.
50 mm ID – Maximum Industrial‑Scale Loading
Provides extremely high sample loading capacity (top tier across entire column system)
Designed for industrial purification, manufacturing workflows, and continuous processing
Supports ultra‑high‑flow chromatography systems
Typical Flow Rate Range
~50–150 mL/min (system dependent)
Optimize based on process requirements and system capability
✅ Designed for process HPLC systems capable of ultra‑high flow rates and very large injection volumes
Flow Rate & System Considerations (50 mm ID, 10 µm, 300 Å)
Requires systems capable of:
Ultra‑high flow rates
Very large injection volumes
Compatible with:
Process chromatography systems
Industrial pumps and injection platforms
Large‑volume fraction collection or continuous processing systems
⚠️ Not suitable for analytical, semi‑prep, or standard preparative HPLC systems
Why Choose Wide Pore (300 Å) C18 (10 µm, Process Scale)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore structures
Process‑Scale Biomolecule Method Development
Ideal for developing and refining purification workflows at industrial scale
Balances throughput with improved separation capability
Supports efficient scale‑up to longer columns and full manufacturing systems
Scalable Workflow Integration
Predictable selectivity across analytical → semi‑prep → prep → production → process
Enables seamless method transfer and industrial scale‑up
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances robustness in high‑load process environments
High Purity Silica
Consistent chromatographic performance
Reduced variability across large‑batch production and continuous runs
Durable & Cost‑Efficient
Long column lifetime
Designed for high‑load industrial workflows
Reduces downtime and operational costs
Typical Applications
Industrial‑scale biomolecule purification
Protein and peptide separations
Process chromatography development
Pharmaceutical manufacturing
Continuous processing systems
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires process‑scale HPLC system capability (50 mm ID)
Ideal for method refinement prior to full production or continuous processing
Optimize for resolution vs throughput depending on manufacturing goals
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable