Prep HPLC Column, RP C18, Wide Pore, 10um, 300A. 30mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68018‑P30‑100‑3M Cogent RP C18 Wide Pore Preparative HPLC Column is designed for production‑scale biomolecule purification and preparative method development, combining 10 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, extremely high loading capacity, and durable chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Consistent and reproducible purification performance
The 300 Å pore structure allows large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å materials.
This column serves as a USP L1 alternate and is widely used in pilot and production‑scale purification workflows.
100 mm Length – Increased Resolution for Production Development
Higher resolving power than 50 mm scouting columns
Improved separation of proteins, peptides, and complex biomolecules
Increased peak capacity for preparative purification workflows
Ideal for refining methods prior to longer columns or production‑level runs
This format is selected when greater resolution is required beyond initial production‑scale screening.
30 mm ID – Maximum Production‑Scale Loading
Provides extremely high sample loading capacity
Designed for large‑scale biomolecule purification and manufacturing workflows
Supports very high‑flow preparative chromatography systems
Typical Flow Rate Range
~20–60 mL/min (system dependent)
Optimize based on instrument capability and production requirements
✅ Designed for production‑scale HPLC systems capable of high flow and large injection volumes
Flow Rate & System Considerations (30 mm ID, 10 µm, 300 Å)
Requires systems capable of:
Very high flow rates
Large injection volumes
Compatible with:
Production preparative pumps
Large‑volume injection systems
High‑capacity fraction collection systems
⚠️ Not intended for analytical or semi‑prep HPLC systems
Why Choose Wide Pore (300 Å) C18 (10 µm, Production Scale)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore structures
Method Development to Production
Ideal for developing and refining biomolecule purification workflows
Predictable selectivity for transition to longer columns and full production scale
Supports efficient workflow continuity across all HPLC scales
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances stability for biomolecule separations
High Purity Silica
Consistent chromatographic performance
Reduced variability across runs and production batches
Durable & Cost‑Efficient
Long column lifetime
Designed for high‑load production workflows
Reduced downtime and maintenance costs
Typical Applications
Production‑scale biomolecule purification
Protein and peptide separations
Preparative method development
High‑throughput pharmaceutical manufacturing
Complex biomolecule mixtures
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires production‑scale HPLC system capability (30 mm ID)
Ideal for method refinement prior to full production runs
Optimize for resolution vs throughput balance
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable