Catalog Number - 68018-P21-PG1-3

The 68018‑P21‑PG1‑3 Cogent RP C18 Wide Pore Prep Guard Column Cartridge is designed to protect preparative HPLC columns used in large‑scale biomolecule separations, extending column life while maintaining consistent chromatographic performance.

Packed with 10 µm Cogent RP C18™ Wide Pore stationary phase (300 Å), and manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding, this cartridge is fully double end‑capped to provide:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Reliable and reproducible chromatographic protection

Constructed in a stainless steel housing with stainless steel frits, this guard cartridge is built for durability in high‑flow, high‑load preparative environments.

Purpose of Guard Columns

• Protect preparative columns from:

  • Particulate contamination

  • Strongly retained biomolecules

  • Matrix buildup and fouling

• Extend column lifetime

• Maintain consistent retention and peak shape

• Reduce downtime and replacement costs

300 Å Wide Pore – Biomolecule Protection

• Designed specifically for large molecular weight analytes

• Ideal for:

  • Proteins

  • Peptides

  • Macromolecules

• Prevents:

  • Pore blocking

  • Restricted diffusion effects associated with 100 Å media

10 µm Guard Media – Preparative Compatibility

• Matches 10 µm preparative column packing

• Supports high‑flow, high‑load purification workflows

• Maintains consistent selectivity during scale‑up

✅ Ensures method integrity from semi‑prep to full preparative scale

21.2 mm ID – Preparative Scale Systems

• Designed for 21.2 mm ID preparative HPLC columns

• Supports large injection volumes and high flow rates

• Maintains performance in large‑scale purification processes

⚠️ Requires use with a 21.2 mm ID prep guard column holder system

Why Choose Wide Pore Guard Cartridges (10 µm, 300 Å)

Matched Chemistry for Biomolecules

• Same wide‑pore C18 chemistry as the column being protected

• Maintains selectivity and retention behavior

• Prevents unintended method changes

Enhanced Protection for Large Molecules

• Traps strongly retained biomolecules before column entry

• Reduces column fouling and degradation

High‑Load Preparative Alignment

• Designed for purification workflows and scale‑up environments

• Supports analytical → semi‑prep → preparative consistency

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances stability for biomolecule separations

High Purity Silica

• Consistent cartridge‑to‑cartridge performance

• Reliable protection across runs

Durable Construction

• Stainless steel housing and frits

• Designed for high‑flow preparative systems

Typical Applications

• Preparative biomolecule purification

• Protein and peptide separations

• Column protection in high‑load workflows

• Method development and scale‑up

• Pharmaceutical and QC processes

• USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for 300 Å wide‑pore biomolecule applications

• Must be used with a compatible 21.2 mm prep guard column holder

• Replace regularly to maintain optimal column performance

• Critical for protecting high‑value preparative columns

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.