Catalog Number - 68018-P21-50-3M

The 68018‑P21‑50‑3M Cogent RP C18 Wide Pore Preparative HPLC Column is designed for biomolecule purification and preparative method development, combining 10 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, high loading capacity, and durable chromatographic performance.

Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:

• Reduced silanol interactions

• Improved peak shape for proteins, peptides, and large polar analytes

• Consistent and reproducible purification performance

The 300 Å pore size enables large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to 100 Å materials.

This column is ideal as a USP L1 alternate for large‑molecule applications and as a fast scouting platform for preparative biomolecule workflows.

50 mm Length – Fast Biomolecule Scouting & Purification

• Designed for rapid separations and short run times

• Ideal for early‑stage biomolecule method development

• Reduces solvent consumption during screening

• Enables high‑throughput preparative workflows

21.2 mm ID – High‑Capacity Preparative Scale

• Provides very high sample loading capacity

• Ideal for large‑scale biomolecule purification and isolation

• Supports high‑flow preparative chromatography workflows

Typical Flow Rate Range

• ~10–30 mL/min (system dependent)

• Optimize based on instrument capability and purification requirements

✅ Designed for preparative HPLC systems capable of high flow rates and large injection volumes

Flow Rate & System Considerations (21.2 mm ID, 10 µm, 300 Å)

• Requires systems capable of:

  • High flow rates

  • Large injection volumes

• Compatible with:

  • Preparative pumps

  • High‑volume injection systems

  • Fraction collection systems

⚠️ Not intended for analytical or semi‑prep HPLC systems

Why Choose Wide Pore (300 Å) C18 (10 µm, Preparative)

Designed for Biomolecules

• Enables purification of:

  • Proteins

  • Peptides

  • Large hydrophobic compounds

• Eliminates mass transfer limitations of smaller pore structures

Ideal for Preparative Scouting

• Used to develop and optimize biomolecule methods prior to scale‑up

• Predictable selectivity for transition to longer columns or production workflows

• Supports efficient analytical → semi‑prep → preparative workflows

Maintains Reversed‑Phase Selectivity

• Retains classic C18 hydrophobic interaction behavior

• Suitable for lipids and long‑chain fatty acids

Cogent RP C18™ Advantages

Fully End‑Capped Surface

• Minimizes secondary interactions

• Improves peak symmetry

• Enhances stability for biomolecule separations

High Purity Silica

• Consistent chromatographic performance

• Reduced variability between runs and batches

Durable & Cost‑Efficient

• Long column lifetime

• Designed for high‑load purification workflows

• Reduced maintenance requirements

Typical Applications

• Preparative biomolecule purification

• Protein and peptide separations

• Preparative method scouting

• Hydrophobic and neutral compound separations

• Analytical to preparative scale‑up workflows

• USP L1 wide‑pore reversed‑phase methods

Important Usage Notes

• Designed for 300 Å wide‑pore biomolecule applications

• Requires preparative HPLC system capability (21.2 mm ID)

• Ideal for fast screening prior to scale‑up or longer column use

• Optimize for throughput vs resolution balance

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

• Orders cannot be canceled once placed

• Custom items are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification:

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.