Prep HPLC Column, RP C18, Wide Pore, 10um, 300A. 21.2mm ID x 100mm Length. Cogent Brand. 1 EA.
Additional Info:
The 68018‑P21‑100‑3M Cogent RP C18 Wide Pore Preparative HPLC Column is designed for biomolecule purification and preparative method development, combining 10 µm particles with a 300 Å wide‑pore structure to deliver enhanced analyte accessibility, high loading capacity, and durable chromatographic performance.
Manufactured using high‑purity silica with traditional octadecylsilane (C18) bonding and fully double end‑capped, this stationary phase provides:
Reduced silanol interactions
Improved peak shape for proteins, peptides, and large polar analytes
Consistent and reproducible purification performance
The 300 Å pore size enables large biomolecules to fully access the stationary phase, minimizing restricted diffusion and improving recovery compared to standard 100 Å media.
This column is ideal as a USP L1 alternate for large‑molecule applications and as a refinement platform for preparative biomolecule workflows.
100 mm Length – Increased Resolution for Biomolecule Development
Higher resolving power than 50 mm preparative scouting columns
Improved separation of proteins, peptides, and complex biomolecule mixtures
Increased peak capacity for purification workflows
Ideal for refining separations prior to longer columns or production‑scale systems
This configuration is selected when greater resolution is required beyond initial preparative screening.
21.2 mm ID – High‑Capacity Preparative Scale
Provides very high sample loading capability
Ideal for large‑scale biomolecule purification and isolation
Supports high‑flow preparative chromatography workflows
Typical Flow Rate Range
~10–30 mL/min (system dependent)
Optimize based on instrument capability and purification goals
✅ Designed for preparative HPLC systems capable of high flow rates and large injection volumes
Flow Rate & System Considerations (21.2 mm ID, 10 µm, 300 Å)
Requires systems capable of:
High flow rates
Large injection volumes
Compatible with:
Preparative pumps
High‑volume injection systems
Fraction collection systems
⚠️ Not intended for analytical or semi‑prep HPLC systems
Why Choose Wide Pore (300 Å) C18 (10 µm, Preparative)
Designed for Biomolecules
Enables purification of:
Proteins
Peptides
Large hydrophobic compounds
Eliminates mass transfer limitations of smaller pore structures
Method Development to Scale‑Up
Ideal for developing and refining biomolecule purification methods
Predictable selectivity for transition to longer columns or production scale
Supports efficient analytical → semi‑prep → preparative workflows
Maintains Reversed‑Phase Selectivity
Retains classic C18 hydrophobic interaction behavior
Suitable for lipids and long‑chain fatty acids
Cogent RP C18™ Advantages
Fully End‑Capped Surface
Minimizes secondary interactions
Improves peak symmetry
Enhances stability for biomolecule separations
High Purity Silica
Consistent chromatographic performance
Reduced variability across runs and batches
Durable & Cost‑Efficient
Long column lifetime
Designed for high‑load purification workflows
Reduced maintenance requirements
Typical Applications
Preparative biomolecule purification
Protein and peptide separations
Method development and optimization
Hydrophobic and neutral compound isolation
Analytical to preparative scale‑up workflows
USP L1 wide‑pore reversed‑phase methods
Important Usage Notes
Designed for 300 Å wide‑pore biomolecule applications
Requires preparative HPLC system capability (21.2 mm ID)
Ideal for method refinement prior to full‑scale purification
Optimize for resolution vs throughput balance
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable